Polymerase chain reaction for detection of Leptospira spp. in clinical samples
| Autor: | P Perolat, I. Saint Girons, P. Amouriaux, Fabrice Merien, G. Baranton |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adult
Male Microbiology (medical) Adolescent Molecular Sequence Data Leptospira interrogans serovar canicola Urine DNA Ribosomal Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Mice Species Specificity Leptospira law RNA Ribosomal 16S Leptospiraceae Animals Humans Polymerase chain reaction Aged Treponema Base Sequence biology Nucleic Acid Hybridization Middle Aged bacterial infections and mycoses biology.organism_classification Virology Blotting Southern Blood Leptospira kirschneri Spirochaetales bacteria Female Leptospira interrogans Borrelia hermsii Digoxigenin Weil Disease Research Article |
| Zdroj: | Journal of Clinical Microbiology. 30:2219-2224 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.30.9.2219-2224.1992 |
| Popis: | A sensitive assay for Leptospira spp., the causative agent of leptospirosis, was developed on the basis of the polymerase chain reaction (PCR). A 331-bp sequence from the Leptospira interrogans serovar canicola rrs (16S) gene was amplified, and the PCR products were analyzed by DNA-DNA hybridization by using a 289-bp fragment internal to the amplified DNA. Specific PCR products also were obtained with DNA from the closely related nonpathogenic Leptospira biflexa but not with DNA from other spirochetes, such as Borrelia burgdorferi, Borrelia hermsii, Treponema denticola, Treponema pallidum, Spirochaeta aurantia, or more distant organisms such as Escherichia coli, Staphylococcus aureus, Mycobacterium tuberculosis, and Proteus mirabilis. The assay was able to detect as few as 10 bacteria. Leptospira DNA was detected in urine from experimentally infected mice. In addition, the test was found to be suitable for diagnosing leptospirosis in humans. Cerebrospinal fluid and urine from patients with leptospirosis were positive, whereas samples from control uninfected patients were negative. |
| Databáze: | OpenAIRE |
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