rrn operons in Haemophilus parainfluenzae and mosaicism of conserved and species-specific sequences in the 16S-23S rDNA long spacer
| Autor: | Viviana Gianninò, Angela Privitera, Oriana Di Marco, Stefania Stefani, Anna Scuto, Maria Santagati, Giancarlo Rappazzo |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Haemophilus Biology rrn operons Microbiology Genome Polymerase Chain Reaction law.invention Plasmid mosaic structure RNA Transfer law Haemophilus parainfluenzae RNA Ribosomal 16S DNA Ribosomal Spacer Cloning Molecular Molecular Biology Ribosomal DNA Polymerase chain reaction Conserved Sequence Genetics Base Sequence Sequence Homology Amino Acid spacer organisation General Medicine Spacer DNA Sequence Analysis DNA Ribosomal RNA biology.organism_classification Molecular biology RNA Transfer Glu Electrophoresis Gel Pulsed-Field Blotting Southern RNA Ribosomal 23S Transfer RNA Plasmids |
| Zdroj: | Research in microbiology. 152(5) |
| ISSN: | 0923-2508 |
| Popis: | The mosaic organisation of short-sequence boxes was analysed in the cloned and sequenced long ribosomal spacer (547 bp) of Haemophilus parainfluenzae GR. Comparison and alignment of both the long and the short spacer were performed in H. parainfluenzae and H. influenzae Rd. The long spacer contained two tRNA genes (tRNA(Ala) and tRNA(Ile)) which are highly homologous to the corresponding genes found in the spacers of other species, such as Haemophilus spp., Actinobacillus spp., and Plesiomonas shigelloides. At the 3' end of tRNA(Ala) a putative ribosomal spacer loop was found, showing a strong secondary structure. Pulsed field gel electrophoresis (PFGE) analysis after restriction of the genome of H. parainfluenzae GR with I-Ceu I and subsequent polymerase chain reaction (PCR) analysis of PFGE-separated DNA fragments demonstrated that the H. parainfluenzae genome contained six operons and that the long spacer was present in three copies of them. Two short DNA segments were identified as being species-specific, allowing us to design PCR primers which were useful in the molecular identification of H. parainfluenzae isolates. |
| Databáze: | OpenAIRE |
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