Morin attenuates osteoclast formation and function by suppressing the NF‐κB , MAPK and calcium signalling pathways

Autor: Yaosen Wu, Yifeng Shi, Yuhan Jiang, Jinghao Lin, Chen Liu, Haiming Jin, Xiang-Yang Wang, Lin Ye, Jiake Xu, Tianchen Qian, Youjin Pan, Shiwei Shen
Rok vydání: 2021
Předmět:
Zdroj: Phytotherapy Research. 35:5694-5707
ISSN: 1099-1573
0951-418X
DOI: 10.1002/ptr.7229
Popis: Morin is a natural compound isolated from moraceae family members and has been reported to possess a range of pharmacological activities. However, the effects of morin on bone-associated disorders and the potential mechanism remain unknown. In this study, we investigated the anti-osteoclastogenic effect of morin in vitro and the potential therapeutic effects on ovariectomy (OVX)-induced osteoporosis in vivo. In vitro, by using a bone marrow macrophage-derived osteoclast culture system, we determined that morin attenuated receptor activator of nuclear factor (NF)-κB ligand (RANKL)-induced osteoclast formation via the inhibition of the mitogen-activated protein kinase (MAPK), NF-κB and calcium pathways. In addition, the subsequent expression of nuclear factor of activated T cells c1 (NFATc1) and c-fos was significantly suppressed by morin. In addition, NFATc1 downregulation led to the reduced expression of osteoclastogenesis-related marker genes, such as V-ATPase-d2 and Integrin β3. In vivo, results provided that morin could effectively attenuate OVX-induced bone loss in C57BL/6 mice. In conclusion, our results demonstrated that morin suppressed RANKL-induced osteoclastogenesis via the NF-κB, MAPK and calcium pathways, in addition, its function of preventing OVX-induced bone loss in vivo, which suggested that morin may be a potential therapeutic agent for postmenopausal osteoporosis treatment.
Databáze: OpenAIRE
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