Alloscardovia macacae sp. nov., isolated from the milk of a macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov
| Autor: | Olga Kofroňová, I. Profousová, Vojtěch Rada, Eva Vlková, Radko Pechar, Jaroslav Havlik, Šárka Ročková, Jan Kopečný, Oldřich Benada, Vera Bunesova, Jiri Killer |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Sequence analysis Molecular Sequence Data Carbohydrates Peptidoglycan Biology Microbiology chemistry.chemical_compound Phylogenetics RNA Ribosomal 16S Animals Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Aldehyde-Lyases Base Composition Strain (chemistry) Phylogenetic tree Fatty Acids Sequence Analysis DNA General Medicine Ribosomal RNA 16S ribosomal RNA Macaca mulatta Bacterial Typing Techniques Actinobacteria Milk chemistry Genes Bacterial Multilocus sequence typing Female Multilocus Sequence Typing |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 63:4439-4446 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.051326-0 |
| Popis: | A novel bacterial strain, designated M8T, was isolated from milk of a female macaque bred in captivity. The strain was Gram-stain-positive, anaerobic, irregular coccoid–rod-shaped without catalase activity. Analysis of 16S rRNA gene sequence similarity revealed that the isolate was most closely related toAlloscardovia omnicolensCCUG 31649T(96.4 %) andMetascardovia cricetiOMB105T(96.6 %). Sequences ofhsp60,fusA, andxfpgenes also confirmed that the strain was most closely related to the type strains ofA. omnicolensandM. criceti. The isolate produced fructose-6-phosphate phosphoketolase which is in agreement with classification within the familyBifidobacteriaceae. The major fatty acids were C18 : 1ω9c(35.8 %), C16 : 1(6.2 %) and C14 : 0(5.7 %). Polar lipid analysis revealed five different glycolipids, two unidentified phospholipids and diphosphatidylglycerol. The peptidoglycan was of the type A4αl-Lys–d-Asp with the presence ofd(l)-alanine,d-glutamine,d-asparagine andl-lysine. The DNA G+C content of strain M8Twas 50.1 mol%. On the basis of genetic, phylogenetic and phenotypic data, strain M8Trepresents a novel species of the genusAlloscardoviafor which the nameAlloscardovia macacaesp. nov. is proposed. The type strain is M8T( = DSM 24762T = CCM 7944T). In addition, our results also revealed thatAlloscardovia omnicolensDSM 21503TandMetascardovia cricetiDSM 17774Tdo not belong to different genera within the familyBifidobacteriaceae. We therefore propose to reclassifyMetascardovia cricetiasAlloscardovia criceticomb. nov. An emended description of the genusAlloscardoviais also provided. |
| Databáze: | OpenAIRE |
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