Immune Cells on the Corneal Endothelium of an Allogeneic Corneal Transplantation Rabbit Model
| Autor: | Nigel J. Fullwood, Elena Koudouna, Ryota Inoue, Yugo Okazaki, Shinichiro Nakano, Naoki Okumura, Noriko Koizumi, Shigeru Kinoshita, Junko Hori |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Graft Rejection Male 0301 basic medicine Corneal endothelium Pathology medicine.medical_specialty genetic structures Endothelium Neutrophils T-Lymphocytes medicine.medical_treatment Cell Count Biology Corneal Diseases 03 medical and health sciences 0302 clinical medicine Immune system Cornea medicine Animals Humans Transplantation Homologous Corneal transplantation Aged Immunity Cellular Macrophages Endothelium Corneal Graft Survival Corneal Transplant Middle Aged eye diseases Transplantation Disease Models Animal 030104 developmental biology medicine.anatomical_structure Immunology Descemet Stripping Endothelial Keratoplasty Disease Progression Microscopy Electron Scanning 030221 ophthalmology & optometry Cytokines Female Rabbits sense organs |
| Zdroj: | Investigative Opthalmology & Visual Science. 58:242 |
| ISSN: | 1552-5783 0146-0404 |
| Popis: | Purpose: Corneal endothelial cell density undergoes a progressive decrease for many years after transplantation, eventually threatening patients with late endothelial failure. The purpose of this study was to investigate the possibility of an immunologic response in successfully grafted corneal endothelium. Methods: The corneal endothelium of patients who had undergone corneal transplantation was evaluated by specular microscopy. Rabbit models were subjected to penetrating keratoplasty (PK) with either syngeneic or allogeneic corneal transplants and Descemet's stripping endothelial keratoplasty (DSEK) with allogeneic corneal transplants. The presence of immune cells and expression of proinflammatory cytokines were determined by immunostaining. The corneal endothelium and immune cells were also evaluated by scanning electron microscopy. Results: Scanning slit contact specular microscopy of patients with no features of graft rejection revealed cell-like white dots on the grafted corneal endothelium. The corneal endothelium of the allogeneic PK and DSEK rabbit models displayed the presence of immune cells, including CD4+ T-helper cells, CD8+ cytotoxic T cells, CD20+ B lymphocytes, CD68+ macrophages, and neutrophils, but these immune cells were rarely observed in the syngeneic PK model. These immune cells also produced proinflammatory cytokines. Notably, some of the corneal endothelial cells situated near these immune cells exhibited features of apoptosis. Conclusions: T lymphocytes, B lymphocytes, macrophages, and neutrophils are present on the grafted corneal endothelium in both PK and DSEK allogeneic rabbit models. The potential involvement of immune cells as an underlying pathophysiology for late endothelial failure deserves further examination. |
| Databáze: | OpenAIRE |
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