Decreased expression and function of adipocyte hormone-sensitive lipase in subcutaneous fat cells of obese subjects
| Autor: | Dominique Langin, Peter Arner, Signy Reynisdottir, Mia Klannemark, Cecilia Holm, Katrin Fredby, Valérie Large |
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| Přispěvatelé: | Karolinska University Hospital [Stockholm], Institut Louis Bugnard, Lund University [Lund], Large, Valerie |
| Rok vydání: | 1999 |
| Předmět: |
Glycerol
Male Adipose tissue Hormone-sensitive lipase Biochemistry Body Mass Index chemistry.chemical_compound 0302 clinical medicine Endocrinology Adipocyte lipase MESH: Obesity Insulin MESH: Cell Size MESH: Aged 0303 health sciences MESH: Sterol Esterase MESH: Middle Aged biology Age Factors Phosphodiesterase Middle Aged MESH: Gene Expression Regulation Adipose Tissue Female catecholamines MESH: Adipose Tissue medicine.drug Adult medicine.medical_specialty MESH: Mutation mRNA Lipolysis enzymes 030209 endocrinology & metabolism QD415-436 MESH: Insulin MESH: Body Mass Index 03 medical and health sciences Sex Factors MESH: Sex Factors MESH: Glycerol Isoprenaline Internal medicine medicine Humans MESH: Lipolysis Body Weights and Measures Obesity RNA Messenger Lipase MESH: Body Weights and Measures 030304 developmental biology MESH: RNA Messenger Aged Cell Size MESH: Age Factors MESH: Humans MESH: Adult Cell Biology Sterol Esterase Enzyme assay MESH: Male [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition chemistry Gene Expression Regulation Mutation biology.protein MESH: Female [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Journal of Lipid Research Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 1999, 40 (11), pp.2059-66 Karolinska Institutet Europe PubMed Central Journal of Lipid Research, Vol 40, Iss 11, Pp 2059-2065 (1999) |
| ISSN: | 0022-2275 |
| Popis: | Decreased lipolytic effect of catecholamines in adipose tissue has repeatedly been demonstrated in obesity and may be a cause of excess accumulation of body fat. However, the mechanisms behind this lipolysis defect are unclear. The role of hormone-sensitive lipase was examined using abdominal subcutaneous adipocytes from 34 obese drug-free and otherwise healthy males or females and 14 non-obese control subjects. The enzyme catalyzes the rate-limiting step of the lipolysis pathway. The maximum lipolytic capacity of fat cells was significantly decreased in obesity when measured using either a non-selective beta-adrenergic receptor agonist (isoprenaline) or a phosphodiesterase resistant cyclic AMP analogue (dibutyryl cyclic AMP). Likewise, enzyme activity, protein expression, and mRNA of hormone-sensitive lipase were significantly decreased in adipocytes of obese subjects. The findings were not influenced by age or gender. The data suggest that a decreased expression of hormone-sensitive lipase in subcutaneous fat cells, which in turn causes decreased enzyme function and impaired lipolytic capacity of adipocytes, is present in obesity. Impaired expression of the hormone-sensitive lipase gene might at least in part explain the enzyme defect.—Large, V., S. Reynisdottir, D. Langin, K. Fredby, M. Klannemark, C. Holm, and P. Arner. Decreased expression and function of adipocyte hormone-sensitive lipase in subcutaneous fat cells of obese subjects. J. Lipid Res. 1999. 40: 2059–2065. |
| Databáze: | OpenAIRE |
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