Comparison of buffy coat preparation to direct method for the evaluation and interpretation of bone marrow aspirates
| Autor: | Jorge A. Ortega, Parvin Izadi, Thomas D. Coates |
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| Rok vydání: | 1993 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry Lymphoblastic Leukemia Biopsy Needle Cytological Techniques Cell Count Hematology Buffy coat Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Buffy Coat Preparation Leukemia medicine.anatomical_structure Bone Marrow hemic and lymphatic diseases Acute lymphocytic leukemia Humans Medicine In patient Direct smear Bone marrow business |
| Zdroj: | American Journal of Hematology. 43:107-109 |
| ISSN: | 1096-8652 0361-8609 |
| DOI: | 10.1002/ajh.2830430207 |
| Popis: | Bone marrow differential and French, American, British (FAB) classification of buffy coat preparation (BCP) was compared to direct method (DM) in 69 pediatric patients with various hematologic and oncologic disorders. The marrow evaluation differed significantly in 12 of 69 patients (17.4%). The differential counts were discordant in 9 out of 69 patients (13%), and the FAB classifications were discordant in 3 out of 25 patients (12%) with acute lymphoblastic leukemia (ALL). Underestimation of the percent blasts occurred with buffy coat preparation in patients with acute non-lymphoblastic leukemia (ANLL). While buffy coat preparations can facilitate morphologic evaluation in marrow malignancies, significant errors can occur in determination of the differential count. Direct smear should be used in conjunction with buffy coat smears in the evaluation of bone marrow aspirates. |
| Databáze: | OpenAIRE |
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