Immune challenge-stimulated hypophysiotropic corticotropin-releasing hormone messenger RNA expression is associated with an induction of neurotensin messenger RNAs without alteration of vasopressin messenger RNAs

Autor: Alain Sarrieau, Christian Juanéda, Marc Corio, Gérard Tramu, P. Lafon-Dubourg, Philippe Ciofi
Přispěvatelé: ProdInra, Migration, Neurogénétique et Stress (NS), Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2
Rok vydání: 1999
Předmět:
Lipopolysaccharides
Male
Hypothalamo-Hypophyseal System
endocrine system
Vasopressin
medicine.medical_specialty
Corticotropin-Releasing Hormone
Vasopressins
[SDV]Life Sciences [q-bio]
Neuropeptide
LIPOPOLYSACCHARIDE
In situ hybridization
Biology
Escherichia coli O157
03 medical and health sciences
chemistry.chemical_compound
Corticotropin-releasing hormone
0302 clinical medicine
ARN-MESSAGER
Internal medicine
Gene expression
medicine
Animals
RNA
Messenger
Rats
Wistar
In Situ Hybridization
Neurotensin
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
Messenger RNA
General Neuroscience
Immunity
RNA Probes
Rats
Endotoxins
[SDV] Life Sciences [q-bio]
Endocrinology
nervous system
chemistry
CRH
Hypothalamus
RAT
Corticosterone
hormones
hormone substitutes
and hormone antagonists
030217 neurology & neurosurgery
Paraventricular Hypothalamic Nucleus
Zdroj: Neuroscience
Neuroscience, Elsevier-International Brain Research Organization, 1999, 93 (1), pp.393-400
ISSN: 0306-4522
1873-7544
Popis: The corticotropin-releasing hormone neurons of the hypothalamic paraventricular nucleus are the final common pathway of the neuroendocrine adaptative response to a variety of stressors. To meet varied homeostatic needs, corticotropin-releasing hormone neurons exhibit a marked phenotypical plasticity, enabling them to rapidly modify their neuroendocrine output. In particular, they synthesize the neuropeptides vasopressin and neurotensin. Under many experimental circumstances, it is observed that corticotropin-releasing hormone and vasopressin are regulated in parallel, whereas the expression of neurotensin seems dissociated, in these neurons, evoking different transcriptional control over the co-existing neuropeptides depending on the adaptative response required. Using radioactive and dual-label in situ hybridization techniques, we have studied the respective expression of paraventricular corticotropin-releasing hormone, vasopressin and neurotensin messenger RNAs in the context of an immune challenge. A single intraperitoneal injection of the endotoxin lipopolysaccharide was administered to adult male rats that were killed 8 h later. Compared to control animals, lipopolysaccharide-injected rats showed elevated plasma corticosterone (614±65 vs 185±40 ng/ml in control) and increased expression of paraventricular corticotropin-releasing hormone messenger RNA (+200%); expression of neurotensin messenger RNA was induced in about one-third of corticotropin-releasing hormone neurons, whereas vasopressin messenger RNA expression remained unchanged. Therefore, in this experimental context and at the time-point examined, co-existing corticotropin-releasing hormone and vasopressin appeared differentially expressed, and an additional stimulus (inflammation) is demonstrated to result in neurotensin expression in neuroendocrine corticotropin-releasing hormone neurons. Neurotensin may be released in the pituitary portal blood to trigger pituitary response associated with mobilization of the immune system.
Databáze: OpenAIRE
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