Mediator links transcription and DNA repair by facilitating Rad2/XPG recruitment
| Autor: | Julie Soutourina, Fanny Eyboulet, Olivier Alibert, Camille Cibot, Thomas Eychenne, Helen Neil, Michel Werner |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Saccharomyces cerevisiae Proteins
DNA Repair Ultraviolet Rays DNA repair RNA polymerase II Saccharomyces cerevisiae Editorials: Cell Cycle Features Radiation Tolerance MED1 Mediator Transcription (biology) yeast Saccharomyces cerevisiae Genetics Humans Promoter Regions Genetic Transcription factor Endodeoxyribonucleases Genome Mediator Complex General transcription factor biology Nuclear Proteins Promoter Endonucleases Rad2/XPG DNA-Binding Proteins biology.protein mediator transcription Gene Deletion Protein Binding Transcription Factors Developmental Biology |
| Zdroj: | Cell Cycle |
| ISSN: | 1549-5477 0890-9369 |
| Popis: | Mediator is a large multiprotein complex conserved in all eukaryotes. The crucial function of Mediator in transcription is now largely established. However, we found that this complex also plays an important role by connecting transcription with DNA repair. We identified a functional contact between the Med17 Mediator subunit and Rad2/XPG, the 3′ endonuclease involved in nucleotide excision DNA repair. Genome-wide location analyses revealed that Rad2 is associated with RNA polymerase II (Pol II)- and Pol III-transcribed genes and telomeric regions in the absence of exogenous genotoxic stress. Rad2 occupancy of Pol II-transcribed genes is transcription-dependent. Genome-wide Rad2 occupancy of class II gene promoters is well correlated with that of Mediator. Furthermore, UV sensitivity of med17 mutants is correlated with reduced Rad2 occupancy of class II genes and concomitant decrease of Mediator interaction with Rad2 protein. Our results suggest that Mediator is involved in DNA repair by facilitating Rad2 recruitment to transcribed genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|