Distant hybrids of Heliocidaris crassispina (♀) and Strongylocentrotus intermedius (♂): identification and mtDNA heteroplasmy analysis
Autor: | Dongyao Cui, Yaqing Chang, Yingying Li, Weijie Zhang, Yaoyao Zhan, Limeng Yang, Jingxian Sun |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Mitochondrial DNA Strongylocentrotus intermedius Evolution 010603 evolutionary biology 01 natural sciences Genome DNA Mitochondrial 03 medical and health sciences Hybrids identification biology.animal QH359-425 Animals Heliocidaris crassispina Anthocidaris Sea urchin Ecology Evolution Behavior and Systematics Hybrid Strongylocentrotus Genetics Distant hybridization biology biology.organism_classification Heteroplasmy 030104 developmental biology Echinoderm Genome Mitochondrial Hybridization Genetic Purebred MtDNA heteroplasmy Research Article |
Zdroj: | BMC Evolutionary Biology, Vol 20, Iss 1, Pp 1-14 (2020) BMC Evolutionary Biology |
ISSN: | 1471-2148 |
Popis: | Background Distant hybridization between the sea urchin Heliocidaris crassispina (♀) and the sea urchin Strongylocentrotus intermedius (♂) was successfully performed under laboratory conditions. A new variety of hybrid sea urchin (HS hybrid) was obtained. However, the early-development success rates for the HS hybrids were significantly lower than those of purebred H. crassispina or S. intermedius offspring. In addition, it was difficult to distinguish the HS-hybrid adults from the pure H. crassispina adults, which might lead to confusion in subsequent breeding attempts. In this study, we attempted to develop a method to quickly and effectively identify HS hybrids, and to preliminarily investigate the molecular mechanisms underlying the poor early-development success rates in the HS hybrids. Results The hybrid sea urchins (HS hybrids) were identified both morphologically and molecularly. There were no significant differences in the test height to test diameter ratios between the HS hybrids and the parents. The number and arrangement of ambulacral pore pairs in the HS hybrids differed from those of the parental lines, which might serve as a useful morphological character for the identification of the HS hybrids. A primer pair that identified the HS hybrids was screened by comparing the mitochondrial genomes of the parental lines. Moreover, paternal leakage induced mitochondrial DNA heteroplasmy in the HS hybrids, which might explain the low rates of early development success in these hybrids. Conclusions The distant-hybrid sea urchins were accurately identified using comparative morphological and molecular genetic methods. The first evidence of mtDNA heteroplasmy after the distant hybridization of an echinoderm was also provided. |
Databáze: | OpenAIRE |
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