Topoisomerase 2 Is Dispensable for the Replication and Segregation of Small Yeast Artificial Chromosomes (YACs)

Autor: María Luisa Martínez-Robles, Estefanía Monturus, Jorge Cebrián, Dora B. Krimer, Jorge Bernardo Schvartzman, Pablo E. Hernández
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Yeast artificial chromosome
DNA Replication
Saccharomyces cerevisiae Proteins
Forms of DNA
Molecular biology
lcsh:Medicine
DNA electrophoresis
Eukaryotic DNA replication
Saccharomyces cerevisiae
Molecular biology assays and analysis techniques
Biochemistry
Molecular Genetics
Saccharomyces
Replication factor C
Control of chromosome duplication
Chromosome Segregation
Molecular Cell Biology
Genetics
lcsh:Science
DNA
Fungal
Chromosomes
Artificial
Yeast
Multidisciplinary
biology
Biology and life sciences
Nucleic acid analysis
DNA
Superhelical
Topoisomerase
lcsh:R
DNA replication
Organisms
Fungi
DNA structure
Genetic Transformation
Cell Biology
DNA
Telomere
Yeast
Molecular biology techniques
DNA Topoisomerases
Type II
biology.protein
DNA supercoil
Origin recognition complex
lcsh:Q
DNA analysis
DNA
Circular
Research Article
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 8, p e104995 (2014)
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1932-6203
Popis: 12 p.-7 fig. Este artículo forma parte de una Tesis Doctoral: https://digital.csic.es/handle/10261/120925
DNA topoisomerases are thought to play a critical role in transcription, replication and recombination as well as in the condensation and segregation of sister duplexes during cell division. Here, we used high-resolution two-dimensional agarose gel electrophoresis to study the replication intermediates and final products of small circular and linear minichromosomes of Saccharomyces cerevisiae in the presence and absence of DNA topoisomerase 2. The results obtained confirmed that whereas for circular minichromosomes, catenated sister duplexes accumulated in the absence of topoisomerase 2, linear YACs were able to replicate and segregate regardless of this topoisomerase. The patterns of replication intermediates for circular and linear YACs displayed significant differences suggesting that DNA supercoiling might play a key role in the modulation of replication fork progression. Altogether, this data supports the notion that for linear chromosomes the torsional tension generated by transcription and replication dissipates freely throughout the telomeres.
This work was sustained by grant BFU2011-22489 to JBS from the Spanish Ministerio de Economía y Competitividad.
Databáze: OpenAIRE
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