Different immunoregulatory components at the decidua basalis of oocyte donation pregnancies
| Autor: | Michael Eikmans, Carin van der Keur, Eileen E L O Lashley, Kim van Bentem, Marie-Louise P. van der Hoorn, Hanneke M. Kapsenberg, Manon Bos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_treatment
Immunoregulatory cytokines Immunology Human leukocyte antigen Fertilization in Vitro Umbilical cord Decidua basalis Andrology Immune system HLA Antigens Pregnancy Placenta Decidua Immunology and Allergy Medicine Humans In vitro fertilisation Oocyte Donation business.industry Histocompatibility Antigens Class I Histocompatibility Antigens Class II FOXP3 Forkhead Transcription Factors General Medicine Oocyte donation pregnancy Regulatory T cells HLA mismatches medicine.anatomical_structure Cytokine embryonic structures Cytokines Female Decidua Basalis business |
| Zdroj: | Human Immunology, 83(4), 319-327. ELSEVIER SCIENCE INC |
| Popis: | Oocyte donation (OD) pregnancies are characterized by more fetal-maternal human leukocyte antigen (HLA) mismatches compared with naturally conceived (NC) and in vitro fertilization (IVF) pregnancies. The maternal immune system has to cope with greater immunogenetic dissimilarity, but involved immunoregulation remains poorly understood. We examined whether the amount of regulatory T cells (Tregs) and immunoregulatory cytokines in decidua basalis of OD pregnancies differs from NC and IVF pregnancies. The cohort included 25 OD, 11 IVF and 16 NC placentas, maternal peripheral blood, and umbilical cord blood of uncomplicated pregnancies. Placenta slides were stained for FOXP3, IL-10, IL-6, gal-1, TGF-0 and Flt-1. Semi-quantitative (FOXP3+ Tregs) and computerized analysis (cytokines) were executed. The blood samples were typed for HLA class I and II to calculate fetal-maternal HLA mismatches. The percentage of Tregs was significantly higher in pregnancies with 4-6 HLA class I mismatches (n = 17), compared to 0-3 mismatches (n = 35; p = 0.04). Cytokine analysis showed significant differences between OD, IVF and NC pregnancies. Flt-1 was significantly lower in pregnancies with 4-6 HLA class I mismatches (p = 0.004), and in pregnancies with 6-10 HLA mismatches in total (p = 0.024). This study suggests that immunoregulation at the fetal-maternal interface in OD pregnancies with more fetal-maternal HLA mismatches is altered.(c) 2021 The Authors. Published by Elsevier Inc. on behalf of American Society for Histocompatibility and Immunogenetics. This is an open access article under the CC BY license (http://creativecommons.org/ licenses/by/4.0/). |
| Databáze: | OpenAIRE |
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