Functional and Safety Aspects of Enterococci Isolated from Different Spanish Foods
| Autor: | Nuha M. K. Yousif, Hikmate Abriouel, Charles M. A. P. Franz, Araceli Castro, Magdalena Martínez-Cañamero, Nabil Ben Omar, Wilhelm H. Holzapfel, Pérez-Pulido Rubén, Antonio Gálvez, Rosario Lucas |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Tetracycline medicine.medical_treatment Enterococcus faecium Colony Count Microbial Dalfopristin Microbial Sensitivity Tests Applied Microbiology and Biotechnology Microbiology Olea RNA Ribosomal 16S Ampicillin Enterococcus faecalis medicine Cluster Analysis Ecology Evolution Behavior and Systematics Antibacterial agent Virulence biology Teicoplanin Quinupristin Nucleic Acid Hybridization biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Random Amplified Polymorphic DNA Technique Meat Products Penicillin Enterococcus Food Microbiology Dairy Products medicine.drug |
| Zdroj: | Systematic and Applied Microbiology. 27:118-130 |
| ISSN: | 0723-2020 |
| DOI: | 10.1078/0723-2020-00248 |
| Popis: | Summary The incidence and diversity of enterococci in retail food samples of meat, dairy and vegetable origin was investigated. Enterococci were present, at concentrations of 10 1 to 10 4 CFU/g. Fifty selected isolates from food samples grouped in two separate clusters by RAPD analysis. Cluster G1 (72% of the isolates) contained the E. faecium CECT 410 T type strain, and also showed a high degree of genetic diversity. Cluster G2 (28% of the isolates) contained the E. faecalis CECT 481 T type strain and was genetically more homogeneous. Virulence traits (haemolysin, gelatinase or DNAse activities, or the presence of structural genes cylL , ace, asa1 and esp ) were not detected. All isolates were sensitive to the antibiotics ampicillin, penicillin, gentamicin, streptomycin and chloramphenicol. A high pecentage of isolates were resistant to erythromycin and rifampicin. Many isolates showed intermediate sensitivity to several antibiotics (tetracycline, ciprofloxacin, levofloxacin, or quinupristin/dalfopristin). Vancomycin and teicoplanin resistance was detected in one strain, but vanA, vanB, vanC1, vanC2 or vanC3 genes were not detected. Many of the isolates showed functional properties of food or health relevance. Production of antimicrobial substances was detected in 17 of the isolates, and 14 of them carried structural genes for enterocins A, B and/or P. |
| Databáze: | OpenAIRE |
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