Cloning and sequence of UK bovine rotavirus gene segment 7: marked sequence homology with simian rotavirus gene segment 8
| Autor: | Ian H. Holmes, Michael L. Dyall-Smith, T.C. Ellemant, Ahmed A. Azad, P.A. Hoyne |
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| Jazyk: | angličtina |
| Rok vydání: | 1983 |
| Předmět: |
Rotavirus
Genes Viral medicine.disease_cause Homology (biology) Gene product Viral Proteins Species Specificity Complementary DNA Genetics medicine Animals Amino Acid Sequence Cloning Molecular Gene Peptide sequence RNA Double-Stranded biology Base Sequence Nucleic acid sequence DNA Haplorhini Molecular biology biology.protein Cattle DNA polymerase I Plasmids |
| Popis: | The genome of the UK bovine rotavirus, which consists of eleven segments of dsRNA was polyadenylated and reverse-transcribed into cDNA. Complementary cDNA strands were annealed and the termini of the duplexes completed using DNA polymerase I. Full-length DNA copies of RNA segments 7, 8 and 9 were cloned into the Pst I site of pBR322 and a clone containing the entire gene 7 was identified and sequenced. Gene 7 is 1059 nucleotides in length and contains a single long open reading frame capable of coding for a protein of 317 amino-acids. The known gene product of segment 7 is a protein with an estimated molecular weight of 33,000 daltons. When the UK bovine rotavirus gene 7 sequence was compared with the published data for the homologous gene (segment 8) of the simian rotavirus SA11, it was found to be identical to it in size and the arrangement of the proposed coding and non-coding regions, and very similar in nucleotide sequence (88% homology). Most of the base changes are silent and the predicted amino-acid sequences are almost identical (96% homology). |
| Databáze: | OpenAIRE |
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