Molecular detection of an importation of type 3 wild poliovirus into Canada from The Netherlands in 1993
| Autor: | Spencer H. S. Lee, K. Fonseca, O. M. Kew, Mick N. Mulders, D. Strong, J. J. Campbell, M. A. Drebot |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Canada Adolescent viruses Molecular Sequence Data Biology medicine.disease_cause Polymerase Chain Reaction Applied Microbiology and Biotechnology Christianity Virus Disease Outbreaks law.invention Capsid law medicine Humans Child Polymerase chain reaction Netherlands Base Sequence Ecology Viral culture Poliovirus Vaccination Outbreak Middle Aged Patient Acceptance of Health Care medicine.disease Virology Poliomyelitis Poliovirus Vaccine Inactivated Child Preschool Enterovirus Capsid Proteins Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 63:519-523 |
| ISSN: | 1098-5336 0099-2240 1992-1993 |
| Popis: | During the fall and winter of 1992-1993 an outbreak of wild poliovirus type 3-associated poliomyelitis involving 71 patients occurred in The Netherlands. Almost all of the individuals involved in the outbreak belonged to an orthodox religious denomination that prohibits vaccination. A surveillance was initiated to determine if there had been an importation of this same strain of wild poliovirus into a southern Alberta community with a similar religious affiliation. Viral culture of stool samples from consenting individuals in the community resulted in viral isolates which typed as poliovirus type 3. Sequencing of amplicons generated from both the 5' nontranslated region and the VP1/2A portion of the genomes from representative poliovirus isolates indicated a greater than 99% genetic similarity to the strain from The Netherlands. The results of this study show that the utilization of PCR-based diagnostics offers an important molecular tool for the concise and rapid surveillance of possible cases of wild poliovirus importation into communities with individuals at risk for infection. |
| Databáze: | OpenAIRE |
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