Characterization and Expression Analysis of Common CarpCyprinus carpioTLR5M
| Autor: | Xiangru Feng, Duo Duan, Qiang Lu, Zhen Sun, Yilong Chen, Shengmei Jia |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Fish Proteins
Lipopolysaccharides Carps DNA Complementary Molecular Sequence Data Mitosis Biology Ligands Evolution Molecular Common carp Rapid amplification of cDNA ends Complementary DNA Gene expression Genetics Animals Amino Acid Sequence Cloning Molecular Carp Molecular Biology Gene Phylogeny Base Sequence Inflammation & Host Response to Infection Cell Membrane Cell Biology General Medicine biology.organism_classification Molecular biology Aeromonas hydrophila Toll-Like Receptor 5 Gene Expression Regulation Organ Specificity TLR5 biology.protein Flagellin |
| Zdroj: | DNA and Cell Biology. 32:611-620 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2013.2051 |
| Popis: | TLR5 is responsible for the recognition of bacterial flagellin in vertebrates. In this study, we cloned the TLR5M gene of common carp using the rapid amplification of cDNA ends (RACE) method. The TLR5M cDNA was 3182 bp in length and contained a 2658-bp open reading frame, which encoded a protein of 885 amino acids (aa). The entire coding region of the TLR5M gene was successfully amplified from genomic DNA and contained a single exon. The aa sequence of carp TLR5M showed the highest similarity (84.46%) to Cirrhinus mrigala. Tissue-specific expression analysis of the TLR5M gene by quantitative real-time polymerase chain reaction revealed its broad distribution in various organs and tissues; however, the highest level of TLR5M expression was noted in the liver. TLR5M gene expression was examined after flagellin stimulation and showed highly significant (p |
| Databáze: | OpenAIRE |
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