Activities of granulocyte-macrophage colony-stimulating factor and interleukin-3 on monocytes
| Autor: | Hidetsugu Mitani, Nobuyuki Minami, Hiroto Araki, Natsuki Hoshino, Naoyuki Katayama, Hiroshi Shiku, Yasushi Ikuta, Hiroyoshi Nishikawa, Atsushi Fujieda, Kazuhiro Nishii, Katsumi Mukai, Hiroyuki Miyashita, Hirohito Suzuki |
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| Rok vydání: | 2004 |
| Předmět: |
T-Lymphocytes
medicine.medical_treatment Lipopolysaccharide Receptors Monoblast Receptor Macrophage Colony-Stimulating Factor Biology Culture Media Serum-Free Monocytes medicine Animals Humans Macrophage Cells Cultured Interleukin 3 U937 cell Tumor Necrosis Factor-alpha Macrophage Colony-Stimulating Factor Macrophages Monocyte Granulocyte-Macrophage Colony-Stimulating Factor Cell Differentiation Dendritic Cells Hematology Flow Cytometry Recombinant Proteins Cell biology medicine.anatomical_structure Granulocyte macrophage colony-stimulating factor Cytokine Immunology Cattle Interleukin-3 Tumor necrosis factor alpha Interleukin-4 medicine.drug |
| Zdroj: | American Journal of Hematology. 75:179-189 |
| ISSN: | 1096-8652 0361-8609 |
| DOI: | 10.1002/ajh.20010 |
| Popis: | We examined the actions of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) on human monocytes, using a serum-free culture system. GM-CSF and IL-3 did not promote the differentiation of monocytes into macrophages but rather into cells with a phenotype compatible with that of immature dendritic cells (DCs). The addition of fetal bovine serum to serum-free cultures with GM-CSF or IL-3 restored the differentiation of monocytes into macrophages. Cells generated with GM-CSF or IL-3 elicited phagocytic activity. Cells generated in the presence of GM-CSF or IL-3, followed by the addition of tumor necrosis factor-alpha, displayed a phenotype of mature DCs, and primed and stimulated immunogenic peptide-specific T lymphocytes. Surprisingly, GM-CSF and IL-3 inhibited macrophage colony-stimulating factor (M-CSF)-dependent differentiation of monocytes into macrophages and induced differentiation into immature DCs. We asked if the inhibition of M-CSF-dependent differentiation into macrophages by GM-CSF or IL-3 was associated with the expression of M-CSF receptors (M-CSFR). GM-CSF or IL-3 down-regulated the expression of M-CSFR. These data demonstrate that GM-CSF and IL-3 primarily support the differentiation of monocytes into DCs and inhibit M-CSF-dependent differentiation into macrophages by suppressing the expression of M-CSFR, thereby promoting differentiation into DCs. |
| Databáze: | OpenAIRE |
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