Interleukin 8 modulates interleukin-1 beta, interleukin-6 and tumor necrosis factor-alpha release from normal human mononuclear cells
| Autor: | Huei Lien Chen, Kuang Hui Sun, Shih Tzu Tsai, Chang-Youh Tsai, Whu Mei Lin, S.-H. Han, Chia-Li Yu, Tang Shueen Liao, Song Chou Shei, Jyh Chiang Wang, Hsin Su Yu |
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| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Enzyme-Linked Immunosorbent Assay In Vitro Techniques Peripheral blood mononuclear cell Proinflammatory cytokine Phagocytosis Internal medicine medicine Cytotoxic T cell Humans Interleukin 8 Cycloheximide Phytohemagglutinins Interleukin 6 Pharmacology biology Dose-Response Relationship Drug business.industry Interleukin-6 Tumor Necrosis Factor-alpha Cell Cycle Interleukin-8 Temperature Interleukin Flow Cytometry Chromatin Recombinant Proteins Endocrinology Cytokine biology.protein Leukocytes Mononuclear Tumor necrosis factor alpha business Interleukin-1 Thymidine |
| Zdroj: | Immunopharmacology. 27(3) |
| ISSN: | 0162-3109 |
| Popis: | Recombinant human interleukin 8 (IL-8) enhanced the release of inflammatory cytokines including interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) from normal human mononuclear cells in a dose-related manner (from 1 ng/ml to 10 ng/ml with a maximal effect at 5 ng/ml) when the cells incubated with IL-8 for 24 h. This cytokine-releasing activity of IL-8 is temperature-dependent and required protein synthesis since low temperature (4 degrees C) and cycloheximide (100 micrograms/ml) minimized the cytokine release from MNC. However, when IL-8 concentration was greater than 20 ng/ml, the cytokine release was suppressed. For further investigating the subcellular mechanism of the adverse effect of high dose IL-8 (20 ng/ml) in cytokine synthesis, human mononuclear cells (1 x 10(6)/ml) were stimulated with PHA (1 microgram/ml) in the presence of 20 ng/ml IL-8 for 3 days. We found not only [3H]thymidine incorporation of MNC was tremendously inhibited but DNA fragmentation appeared. Subsequently, the cell cycle of PHA-stimulated MNC retarded in the phase of G0/G1. These results suggest that in low concentration (5-10 ng/ml) IL-8 not only activated neutrophil phagocytosis but facilitated the release of inflammatory cytokines from mononuclear cells. Higher dose of IL-8 (more than 20 ng/ml) conversely suppressed these cytokine release from damaged cells by its cytotoxic effect. This newly found cytokine-releasing activity of IL-8 may play a role in the modulation of inflammation. |
| Databáze: | OpenAIRE |
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