Effect of eotaxin on the generationof reactive oxygen species from eosinophil cell line, YY-1
| Autor: | Norihoro Saito, Hiroyuki Kayaba, Yoshiyuki Yamada, Hajime Oyamada, Kohei Honda, Yoshimi Kobayashi, Susumu Tsurufuji, Junichi Chihara, Tomoe Kuwasaki, Yumiko Kamada, Chang-Hao Cui |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Eotaxin
Chemokine CCL11 Chemokine Chemotactic Factors Eosinophil Immunology medicine.disease_cause Allergic inflammation Cell Line Eosinophil activation medicine Immunology and Allergy Humans chemistry.chemical_classification Reactive oxygen species biology Chemistry General Medicine respiratory system Eosinophil Eosinophils medicine.anatomical_structure Chemokines CC biology.protein Eosinophil chemotaxis Cytokines Reactive Oxygen Species Oxidative stress |
| Zdroj: | International archives of allergy and immunology. 120 |
| ISSN: | 1018-2438 |
| Popis: | Background: The CC chemokine eotaxin is a selective chemoattractant for eosinophils. Eosinophils have been considered to be the major effector cells in allergic inflammation, since not only eosinophil-specific granule proteins but also reactive oxygen species (ROS) from eosinophils may cause the damage to the cells or tissue of the mucosal epithelium. In this study, we examined the effect of eotaxin on ROS from an eosinophil cell line, YY-1. Methods: ROS in luminol-dependent reaction were examined. Calcium ionophore A23187 were added to the mixture of YY-1 cells with luminol, and then ROS were determined. Results: Eotaxin primed the production of ROS from YY-1 cells. ROS from untreated YY-1 cells evoked with calcium ionophore A23187 in luminol-dependent chemiluminescence gave a maximal value of 1,928 ± 223 intensity counts (IC; mean ± SE, n = 4) and an integral value of 17.04 ± 1.51 IC (×10–4), while eosinophils that were treated with eotaxin gave a maximal value of 2,264 ± 86 IC (10 nM), 2,691 ± 124 IC (100 nM) and an integral value of 21.22 ± 0.67 IC (×10–4; 10 nM), 26.20 ± 1.41 IC (×10–4; 100 nM). Conclusion: Eotaxin might play important roles in the pathogenesis of allergic inflammation through eosinophil activation by priming of eosinophil oxidative metabolism as well as involvement in selective eosinophil chemotaxis. |
| Databáze: | OpenAIRE |
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