A New Master Donor Virus for the Development of Live-Attenuated Influenza B Virus Vaccines
| Autor: | Aitor Nogales, Daniel R. Perez, Chantelle L White, Kevin Chiem, Luis Martinez-Sobrido, Stivalis Cardenas Garcia, Jefferson Santos |
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| Přispěvatelé: | White, CL [0000-0003-2875-4691], Chiem, K [0000-0002-3892-5944], Perez, DR [0000-0002-6569-5689], Santos, J [0000-0001-5895-4163], Garcia, SC [0000-0002-7613-8642], Nogales, A [0000-0002-2424-7900], Martinez-Sobrido, L [0000-0001-7084-0804], White, CL, Chiem, K, Perez, DR, Santos, J, Garcia, SC, Nogales, A, Martinez-Sobrido, L |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
viruses immunogenicity Virus Replication Epitope Madin Darby Canine Kidney Cells Mice hemic and lymphatic diseases Live attenuated influenza vaccine Vaccines Immunogenicity Vaccination Temperature virus diseases vaccines Adaptation Physiological influenza B virus Phenotype QR1-502 Temperature sensitive Infectious Diseases Cold-adapted Influenza Vaccines Female Safety influenza safety animal structures attenuated cold-adapted 030106 microbiology Biology Vaccines Attenuated Microbiology Article Virus Cold adapted Viral Proteins 03 medical and health sciences Dogs Virology Protection efficacy Influenza Human Animals Humans live-attenuated influenza vaccines temperature sensitive Master donor virus Reverse Genetics Influenza Mice Inbred C57BL master donor virus Influenza B virus HEK293 Cells 030104 developmental biology Attenuated Live-attenuated influenza vaccines Mutation protection efficacy |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Viruses Volume 13 Issue 7 Viruses, Vol 13, Iss 1278, p 1278 (2021) |
| Popis: | Influenza B viruses (IBV) circulate annually, with young children, the elderly and immunocompromised individuals being at high risk. Yearly vaccinations are recommended to protect against seasonally influenza viruses, including IBV. Live attenuated influenza vaccines (LAIV) provide the unique opportunity for direct exposure to the antigenically variable surface glycoproteins as well as the more conserved internal components. Ideally, LAIV Master Donor Viruses (MDV) should accurately reflect seasonal influenza strains. Unfortunately, the continuous evolution of IBV have led to significant changes in conserved epitopes compared to the IBV MDV based on B/Ann Arbor/1/1966 strain. Here, we propose a recent influenza B/Brisbane/60/2008 as an efficacious MDV alternative, as its internal viral proteins more accurately reflect those of circulating IBV strains. We introduced the mutations responsible for the temperature sensitive (ts), cold adapted (ca) and attenuated (att) phenotype of B/Ann Arbor/1/1966 MDV LAIV into B/Brisbane/60/2008 to generate a new MDV LAIV. In vitro and in vivo analysis demonstrated that the mutations responsible of the ts, ca, and att phenotype of B/Ann Arbor/1/1966 MDV LAIV were able to infer the same phenotype to B/Brisbane/60/2008, demonstrating its potential as a new MDV for the development of LAIV to protect against contemporary IBV strains. This research was partially funded by the New York Influenza Center of Excellence (NYICE) (NIH 272201400005C), a member of the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Department of Health and Human Services, Centers of Excellence Viruses 2021, 13, 1278 14 of 18 for Influenza Research and Surveillance (CEIRS) contract No. HHSN272201400005C (NYICE) and by the Department of Defense (DoD) Peer Reviewed Medical Research Program (PRMRP) grant W81XWH-18-1-0460-PRMRP-DA and NIH RO1 AI145332-01 to L.M.-S. A.N. received a “Ramon y Cajal” Incorporation grant (RYC-2017) from Spanish Ministry of Science, Innovation. This study was in part supported by a subcontract from the Center for Research on Influenza Pathogenesis (CRIP) under contract HHSN272201400008C from the National Institute of Allergy and Infectious Diseases (NIAID) Centers for Influenza Research and Surveillance (CEIRS) and grant 1R21AI146448-01 from NIAID to D.R.P. D.R.P. receives additional support from the Georgia Research Alliance and from the Caswell S. Eidson endowment funds |
| Databáze: | OpenAIRE |
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