Inhibition of P-selectin-mediated cell adhesion by a sulfated derivative of sialic acid
| Autor: | Tsutomu Tsuji, Tomonori Shodai, Sanae Kudo, Saotomo Itoh, Hajime Shimazu, Masaki Terada, Shuji Fujita, Junsuke Suzuki |
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| Rok vydání: | 2003 |
| Předmět: |
P-selectin
Neutrophils Biophysics HL-60 Cells CHO Cells Biology Biochemistry Neutrophil Activation chemistry.chemical_compound Cricetulus Platelet Adhesiveness Cricetinae Cell Adhesion Animals Humans Tyrosine Cell adhesion Molecular Biology Dose-Response Relationship Drug Cell adhesion molecule Tumor Necrosis Factor-alpha Chinese hamster ovary cell Soluble cell adhesion molecules Cell Biology Platelet Activation Molecular biology Lipids N-Acetylneuraminic Acid Sialic acid P-Selectin chemistry Leukocytes Mononuclear Tumor necrosis factor alpha Reactive Oxygen Species |
| Zdroj: | Biochemical and biophysical research communications. 312(3) |
| ISSN: | 0006-291X |
| Popis: | P-selectin, a carbohydrate-binding cell adhesion molecule expressed on activated endothelial cells and platelets, plays a key role in the recruitment of leukocytes to inflammatory and hemorrhagic sites. It simultaneously recognizes a sialic acid-containing carbohydrate chain and the sulfated tyrosine residues of a specific counter-receptor expressed on the leukocyte surface. We examined the inhibitory effects of a synthetic sulfated derivative of sialic acid (NMSO3) on P-selectin-mediated cell adhesion and found the following: (1) P-selectin/IgG chimera bound to immobilized NMSO3. (2) The binding of P-selectin/IgG chimera to purified P-selectin glycoprotein ligand-1 was inhibited by soluble NMSO3. (3) The adhesion of HL60 cells to P-selectin-expressing CHO cells was inhibited by NMSO3. (4) NMSO3 inhibited P-selectin-induced tumor necrosis factor-alpha production in monocytes and activated platelet-induced generation of reactive oxygen species in neutrophils. In conclusion, NMSO3 acts as a specific inhibitor for P-selectin-mediated cell adhesion and for adhesion-dependent leukocyte activation. |
| Databáze: | OpenAIRE |
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