Characteristic of Pseudomonas syringae pv. atrofaciens Isolated from Weeds of Wheat Field
| Autor: | Yuliia Kolomiiets, Dariusz Suszanowicz, Antonina Kalinichenko, Monika Sporek, Lidiia Pasichnyk, Liudmyla Butsenko, V.P. Patyka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology Virulence lcsh:Technology fatty acids law.invention lcsh:Chemistry Pseudomonas syringae pv. atrofaciens 03 medical and health sciences law RAPD wheat Botany Pseudomonas syringae weeds basal glume rot General Materials Science Instrumentation lcsh:QH301-705.5 Polymerase chain reaction Fluid Flow and Transfer Processes Pseudomonas syringae pv.atrofaciens biology Inoculation lcsh:T Process Chemistry and Technology Glume fungi General Engineering food and beverages phenotypic and genotypic properties biology.organism_classification lcsh:QC1-999 Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 Weed lcsh:Engineering (General). Civil engineering (General) Bacteria lcsh:Physics |
| Zdroj: | Applied Sciences Volume 11 Issue 1 Applied Sciences, Vol 11, Iss 286, p 286 (2021) |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app11010286 |
| Popis: | The aim of this study was the identification of the causative agent of the basal glume rot of wheat Pseudomonas syringae pv. atrofaciens from the affected weeds in wheat crops, and determination of its virulent properties. Isolation of P. syringae pv. atrofaciens from weeds of wheat crops was carried out by classical microbiological methods. To identify isolated bacteria, their morphological, cultural, biochemical, and serological properties as well as fatty acids and Random Amplification of Polymorphic DNA (RAPD)-PCR (Polymerase chain reaction) profiles with the OPA-13 primer were studied. Pathogenic properties were investigated by artificial inoculation of wheat plants and weed plants, from which bacteria were isolated. For the first time, bacteria that are virulent both for weeds and wheat were isolated from weeds growing in wheat crops. It was shown that the fatty acids profiles of the bacteria isolated from the weeds contained typical for P. syringae pv. atrofaciens fatty acids, in particular, hydroxy acids: 3-hydroxydecanoic, 2-hydroxydodecanoic, and 3-hydroxydodecanoic. RAPD-PCR profiles of the newly isolated strains were identical to those of the collection strains P. syringae pv. atrofaciens UCM B-1011 and P. syringae pv. atrofaciens UCM B-1014 and contained a dominant fragment of 700 bp. The isolated strains, according to their phenotypic and genotypic properties, were identified as P. syringae pv. atrofaciens. It was established that the causative agent of basal glume rot of wheat P. syringae pv. atrofaciens is polyphagous and capable of infecting a wide range of plants. The main control measure for cereals diseases caused by P. syringae pv. Atrofaciens&mdash crop rotations with nonhost species, should be revised, and alternative control methods must be proposed. |
| Databáze: | OpenAIRE |
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