Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions
| Autor: | Douglas Law, Babul Airianah Othman, Sharifah Sakinah Syed Alwi, Muhammad Shahid, Ahmed Abdulkareem Najm, Ahmad Azfaralariff, Nahid Khalili, Herryawan Ryadi Eziwar Dyari, Shazrul Fazry |
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| Rok vydání: | 2021 |
| Předmět: |
Cell biology
Molecular biology Science Antimicrobial peptides Antineoplastic Agents Apoptosis Breast Neoplasms Anabas testudineus Article Inhibitory Concentration 50 Cell Line Tumor Protein Interaction Mapping medicine Animals Humans Cytotoxic T cell Cancer Cell Proliferation Multidisciplinary biology Drug discovery Caspase 3 Chemistry Gene Expression Profiling Cell Cycle Fishes Computational Biology Cell Cycle Checkpoints Antimicrobial biology.organism_classification medicine.disease Mucus Biochemistry MCF-7 Cells Medicine Female Drug Screening Assays Antitumor Peptides Antibacterial activity Antimicrobial Peptides |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-20 (2021) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-02007-6 |
| Popis: | Previous study has shown the antimicrobial activities of mucus protein extracted from Anabastestudineus. In this study, we are interested in characterizing the anticancer activity of the A.testudineus antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC50 for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides. |
| Databáze: | OpenAIRE |
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