Multiple clades of Husavirus in South America revealed by next generation sequencing

Autor: Adriana Luchs, Endrya do Socorro Fôro Ramos, Antonio Charlys da Costa, Eric Delwart, Ramendra Pati Pandey, Flavio Augusto de Pádua Milagres, Geovani de Oliveira Ribeiro, V. Samuel Raj, Ulisses Alves Rosa, Emerson Luiz Lima Araújo, Rafael Brustulin, Fabiola Villanova, Élcio Leal, Xutao Deng, Vanessa dos Santos Morais, Ester Cerdeira Sabino
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RNA viruses
Rotavirus
Male
Pathology and Laboratory Medicine
medicine.disease_cause
Genome
Geographical locations
Feces
Reoviruses
Medicine and Health Sciences
Clade
Data Management
Multidisciplinary
biology
Software Engineering
Phylogenetic Analysis
Genomics
Phylogenetics
Medical Microbiology
Viral Pathogens
Child
Preschool
Viruses
Engineering and Technology
Medicine
Female
Pathogens
Brazil
Research Article
Diarrhea
Computer and Information Sciences
Science
Zoology
Gastroenterology and Hepatology
Genome
Viral
Microbiology
DNA sequencing
Virus
Computer Software
Signs and Symptoms
Genetics
medicine
Humans
Evolutionary Systematics
Microbial Pathogens
Taxonomy
Positive-Strand RNA Viruses
Evolutionary Biology
Picornaviridae Infections
Organisms
Biology and Life Sciences
Genetic Variation
Infant
South America
biology.organism_classification
Picornavirales
People and places
Clinical Medicine
Zdroj: PLoS ONE, Vol 16, Iss 3, p e0248486 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Husavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs’ nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study.
Databáze: OpenAIRE
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