Multiple clades of Husavirus in South America revealed by next generation sequencing
Autor: | Adriana Luchs, Endrya do Socorro Fôro Ramos, Antonio Charlys da Costa, Eric Delwart, Ramendra Pati Pandey, Flavio Augusto de Pádua Milagres, Geovani de Oliveira Ribeiro, V. Samuel Raj, Ulisses Alves Rosa, Emerson Luiz Lima Araújo, Rafael Brustulin, Fabiola Villanova, Élcio Leal, Xutao Deng, Vanessa dos Santos Morais, Ester Cerdeira Sabino |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
RNA viruses
Rotavirus Male Pathology and Laboratory Medicine medicine.disease_cause Genome Geographical locations Feces Reoviruses Medicine and Health Sciences Clade Data Management Multidisciplinary biology Software Engineering Phylogenetic Analysis Genomics Phylogenetics Medical Microbiology Viral Pathogens Child Preschool Viruses Engineering and Technology Medicine Female Pathogens Brazil Research Article Diarrhea Computer and Information Sciences Science Zoology Gastroenterology and Hepatology Genome Viral Microbiology DNA sequencing Virus Computer Software Signs and Symptoms Genetics medicine Humans Evolutionary Systematics Microbial Pathogens Taxonomy Positive-Strand RNA Viruses Evolutionary Biology Picornaviridae Infections Organisms Biology and Life Sciences Genetic Variation Infant South America biology.organism_classification Picornavirales People and places Clinical Medicine |
Zdroj: | PLoS ONE, Vol 16, Iss 3, p e0248486 (2021) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Husavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs’ nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study. |
Databáze: | OpenAIRE |
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