Precolumn fluorescence derivatization of the antagonist [Arg6,D-Trp7,9,MePhe8]-substance P[6-11] with benzoin in high-performance liquid chromatography and selective detection of arginine-containing fragments in its degradation products
| Autor: | Auke Bult, Hua Cui, J.Léon E. Reubsaet |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Arginine
Molecular Sequence Data Substance P Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Benzoin Amino Acid Sequence Derivatization Chromatography High Pressure Liquid Fluorescent Dyes Detection limit Chromatography Organic Chemistry Antagonist General Medicine Fluorescence Peptide Fragments Spectrometry Fluorescence chemistry Indicators and Reagents Spectrophotometry Ultraviolet Quantitative analysis (chemistry) |
| Zdroj: | Journal of chromatography. A. 736(1-2) |
| ISSN: | 0021-9673 |
| Popis: | Precolumn fluorescence derivatization for the determination of the antagonist [Arg6, d -Trp7,9,MePhe8]-Substance P 6–11 (antagonist G) using benzoin in HPLC was studied. Under the conditions chosen (0.067 M NaOH, heating at 100°C for 10 s), a good yield of fluorescent derivatives was obtained and no methodology-related degradation occurred. The detection limit of antagonist G was 0.21 nmol/ml. The method has been applied to the selective and sensitive detection of arginine-containing fragments in degradation products of antagonist G. |
| Databáze: | OpenAIRE |
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