The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family: comparative promoter activity of six TaLtp genes in transgenic rice

Autor: Donaldo Meynard, Marie-Françoise Gautier, Philippe Joudrier, Freddy Boutrot, Emmanuel Guiderdoni
Přispěvatelé: Polymorphismes d'intérêt agronomique (UMR PIA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)
Rok vydání: 2006
Předmět:
0106 biological sciences
Plant Science
Plant Roots
01 natural sciences
F30 - Génétique et amélioration des plantes
PROMOTER ANALYSIS
GENE REGULATION
Genes
Reporter
Expression des gènes
Fluorometry
Promoter Regions
Genetic
Triticum
Glucuronidase
2. Zero hunger
Regulation of gene expression
Genetics
0303 health sciences
Genetic transfer
food and beverages
Plants
Genetically Modified
Immunohistochemistry
Multigene Family
Seeds
UidA REPORTER GENE
Molecular Sequence Data
Triticum aestivum
WHEAT
Oryza sativa
Flowers
Biology
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
Transformation
Genetic
Gene family
Amino Acid Sequence
TRANSGENIC RICE
Gene
Transfert de gène
030304 developmental biology
Génie génétique
Reporter gene
Sequence Homology
Amino Acid
Oryza
Promoter
Sequence Analysis
DNA
Scutellum
Plante transgénique
Genetically modified rice
Gène
NON-SPECIFIC LIPID TRANSFER PROTEIN GENES
Carrier Proteins
RIZ
010606 plant biology & botany
Zdroj: Planta
Planta, Springer Verlag, 2007, 225 (4), pp.843-862. ⟨10.1007/s00425-006-0397-7⟩
ISSN: 1432-2048
0032-0935
Popis: UMR DAP - Equipe DGB; International audience; Plant non-specific lipid transfer proteins (nsLTPs) are encoded by a multigene family and support physiological functions, which remain unclear. We adapted an efficient ligation-mediated polymerase chain reaction (LM-PCR) procedure that enabled isolation of 22 novel Triticum aestivum nsLtp (TaLtp) genes encoding types 1 and 2 nsLTPs. A phylogenetic tree clustered the wheat nsLTPs into ten subfamilies comprising 1-7 members. We also studied the activity of four type 1 and two type 2 TaLtp gene promoters in transgenic rice using the beta-Glucuronidase reporter gene. The activities of the six promoters displayed both overlapping and distinct features in rice. In vegetative organs, these promoters were active in leaves and root vascular tissues while no beta-Glucuronidase (GUS) activity was detected in stems. In flowers, the GUS activity driven by the TaLtp7.2a, TaLtp9.1a, TaLtp9.2d, and TaLtp9.3e gene promoters was associated with vascular tissues in glumes and in the extremities of anther filaments whereas only the TaLtp9.4a gene promoter was active in anther epidermal cells. In developing grains, GUS activity and GUS immunolocalization data evidenced complex patterns of activity of the TaLtp7.1a, TaLtp9.2d, and TaLtp9.4a gene promoters in embryo scutellum and in the grain epicarp cell layer. In contrast, GUS activity driven by TaLtp7.2a, TaLtp9.1a, and TaLtp9.3e promoters was restricted to the vascular bundle of the embryo scutellum. This diversity of TaLtp gene promoter activity supports the hypothesis that the encoded TaLTPs possess distinct functions in planta
Databáze: OpenAIRE
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