Transcription factor NFY globally represses the expression of the C. elegans Hox gene Abdominal-B homolog egl-5
| Autor: | Hansong Deng, Yinyan Sun, Yuxia Zhang, Hong Zhang, Libo Yan, Xin Luo, Wenru Hou, E Tian, Jinghua Han, Yaoyao Chen |
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| Rok vydání: | 2006 |
| Předmět: |
animal structures
CAAT box Biology CCAAT box Animals Genetically Modified Expression pattern Transcription (biology) Heterotrimeric G protein Animals NFY Hox gene Caenorhabditis elegans Caenorhabditis elegans Proteins Promoter Regions Genetic Molecular Biology Transcription factor Derepression Genes Helminth Genetics Homeodomain Proteins Genome Helminth Base Sequence egl-5 fungi Genes Homeobox Gene Expression Regulation Developmental Cell Biology DNA Helminth Repressor Proteins CCAAT-Binding Factor embryonic structures Mutation C. elegans Ectopic expression Transcription Developmental Biology Transcription Factors |
| Zdroj: | Developmental biology. 308(2) |
| ISSN: | 0012-1606 |
| Popis: | The C. elegans Hox gene egl-5 (ortholog of Drosophila Abdominal-B) is expressed in multiple tissues in the tail region and is involved in tail patterning. In this study, we identify and clone the corresponding C. elegans orthologs of the components of the heterotrimeric transcription factor NFY, nfya-1, nfyb-1 and nfyc-1 and demonstrate that mutations in these components result in the ectopic expression of egl-5 outside of its normal expression domains. The NFYA-1 protein forms a complex with NFYB-1 and NFYC-1, specifically recognizing the CCAAT box. Mutating a CCAAT box in the proximal promoter of egl-5 also leads to the derepression of egl-5, suggesting a direct role for the NFY complex in the regulation of egl-5. In addition, we show that the NFY complex interacts with the MES-2/MES-6 PcG complex in Hox gene regulation. Thus, our studies unravel a physiological function of NFY in establishing the spatially restricted expression pattern of egl-5. |
| Databáze: | OpenAIRE |
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