Persistent expression of activated notch in the developing hypothalamus affects survival of pituitary progenitors and alters pituitary structure
| Autor: | Paven K. Aujla, George T. Naratadam, Vedran Bogdanovic, Lori T. Raetzman |
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| Rok vydání: | 2015 |
| Předmět: |
endocrine system
Pituitary gland medicine.medical_specialty Notch signaling pathway HES5 Hypothalamus In situ hybridization Biology Article Mice Internal medicine medicine Basic Helix-Loop-Helix Transcription Factors Animals HES1 In Situ Hybridization Homeodomain Proteins Receptors Notch RBPJ Gene Expression Regulation Developmental Immunohistochemistry Repressor Proteins medicine.anatomical_structure Endocrinology Pituitary Gland Transcription Factor HES-1 Signal transduction Developmental Biology Signal Transduction |
| Zdroj: | Developmental dynamics : an official publication of the American Association of Anatomists. 244(8) |
| ISSN: | 1097-0177 |
| Popis: | Background: As the pituitary gland develops, signals from the hypothalamus are necessary for pituitary induction and expansion. Little is known about the control of cues that regulate early signaling between the two structures. Ligands and receptors of the Notch signaling pathway are found in both the hypothalamus and Rathke's pouch. The downstream Notch effector gene Hes1 is required for proper pituitary formation; however, these effects could be due to the action of Hes1 in the hypothalamus, Rathke's pouch, or both. To determine the contribution of hypothalamic Notch signaling to pituitary organogenesis, we used mice with loss and gain of Notch function within the developing hypothalamus. Results: We demonstrate that loss of Notch signaling by conditional deletion of Rbpj in the hypothalamus does not affect expression of Hes1 within the posterior hypothalamus or expression of Hes5. In contrast, expression of activated Notch within the hypothalamus results in ectopic Hes5 expression and increased Hes1 expression, which is sufficient to disrupt pituitary development and postnatal expansion. Conclusions: Taken together, our results indicate that Rbpj-dependent Notch signaling within the developing hypothalamus is not necessary for pituitary development, but persistent Notch signaling and ectopic Hes5 expression in hypothalamic progenitors affects pituitary induction and expansion. Developmental Dynamics 244:921–934, 2015. © 2015 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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