Gill microbiome structure and function in the chemosymbiotic coastal lucinidStewartia floridana
| Autor: | Annette Summers Engel, Danielle E. Gill, Brenton G. Davis, John R. Swetenburg, Shen Jean Lim, Laurie C. Anderson, Barbara J. Campbell |
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| Rok vydání: | 2021 |
| Předmět: |
Gills
0301 basic medicine Gill animal structures Zoology Biology Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Symbiosis Animals Microbiome Phylogeny Chemosynthesis Bacteria 030102 biochemistry & molecular biology Ecology Host (biology) Spirochaeta Microbiota fungi biology.organism_classification Stewartia floridana Bivalvia 030104 developmental biology Metagenomics |
| Zdroj: | FEMS Microbiology Ecology. 97 |
| ISSN: | 1574-6941 0168-6496 |
| DOI: | 10.1093/femsec/fiab042 |
| Popis: | Lucinid bivalves harbor environmentally acquired, chemosynthetic, gammaproteobacterial gill endosymbionts. Lucinid gill microbiomes, which may contain other gammaproteobacterial and/or spirochete taxa, remain under-sampled. To understand inter-host variability of the lucinid gill microbiome, specifically in the bacterial communities, we analyzed the microbiome content of Stewartia floridana collected from Florida. Sampled gills contained a monospecific gammaproteobacterial endosymbiont expressing lithoautotrophic, mixotrophic, diazotrophic and C1 compound oxidation-related functions previously characterized in similar lucinid species. Another low-abundance Spirochaeta-like species in ∼72% of the sampled gills was most closely related to Spirochaeta-like species in another lucinid Phacoides pectinatus and formed a clade with known marine Spirochaeta symbionts. The spirochete expressed genes were involved in heterotrophy and the transport of sugars, amino acids, peptides and other substrates. Few muscular and neurofilament genes from the host and none from the gammaproteobacterial and spirochete symbionts were differentially expressed among quadrats predominantly covered with seagrass species or 80% bare sand. Our results suggest that spirochetes are facultatively associated with S. floridana, with potential scavenging and nutrient cycling roles. Expressed stress- and defense-related functions in the host and symbionts also suggest species–species communications, which highlight the need for further study of the interactions among lucinid hosts, their microbiomes and their environment. |
| Databáze: | OpenAIRE |
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