Exploration of biomarkers from a pilot weight management study for men undergoing radical prostatectomy
| Autor: | James R. Hébert, Harsh B. Pathak, Prabhakar Chalise, William P. Parker, Jill Hamilton-Reeves, Mohamad Dave Dimachkie, John DiGiovanni, Hilary L. Robertson, Carrie Michel, Debra K. Sullivan, Andrew K. Godwin, Misty D. Bechtel, Nitin Shivappa, Eugene K. Lee, J. Brantley Thrasher |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Oncology
Male medicine.medical_specialty Urology medicine.medical_treatment 030232 urology & nephrology Pilot Projects Overweight medicine.disease_cause Article Body Mass Index 03 medical and health sciences Prostate cancer 0302 clinical medicine Weight loss Internal medicine Weight management Biopsy Weight Loss medicine Humans Clinical significance Prospective Studies Prostatectomy medicine.diagnostic_test business.industry Prostatic Neoplasms Immune dysregulation Middle Aged medicine.disease Prognosis 030220 oncology & carcinogenesis medicine.symptom business Biomarkers Diet Therapy Follow-Up Studies |
| Zdroj: | Urol Oncol |
| ISSN: | 1873-2496 |
| Popis: | BACKGROUND: Several biologic mechanisms, including inflammation and immune changes, have been proposed to explain the role of obesity in prostate cancer (PCa) progression. Compared to men of a healthy weight, overweight and obese men are more likely to have PCa recurrence post-prostatectomy. Obesity is related to inflammation and immune dysregulation; thus, weight loss may be an avenue to reduce inflammation and reverse these immune processes. OBJECTIVES: This study explores the reversibility of the biological mechanisms through intentional weight loss using a comprehensive weight management program in men undergoing prostatectomy. Outcomes include blood and tissue biomarkers, microtumor environment gene expression, inflammation markers and Dietary Inflammatory Index (DII) scores. METHODS: Twenty overweight men undergoing prostatectomy participated in this study. Fifteen men chose the intervention and five men chose the non-intervention group. The intervention consisted of a comprehensive weight loss program prior to prostatectomy and a weight maintenance program following surgery. Prostate tissue samples were obtained from diagnostic biopsies before the intervention and prostatectomy samples after weight loss. Blood samples and diet records were collected at baseline, pre-surgery after weight loss and at study end after weight maintenance. Immunohistochemistry (IHC) and NanoString analysis were used to analyze the tissue samples. Flow cytometry was used to assess circulating immune markers. Inflammation markers were measured using Luminex panels. RESULTS: The intervention group lost >5% body weight prior to surgery. DII scores improved during the weight loss intervention from baseline to pre-surgery (P=0.002); and between group differences were significant (P=0.02). DII scores were not associated with IL-6 nor hsCRP. In the intervention, CXCL12, CXCR7 and CXCR4 (C-X-C motif chemokine ligand/receptor) and Ki67 expression decreased in the prostate tissue from biopsy to surgery (P=0.06), yet plasma CXCL12 increased during the same timeframe (P=0.009). The downregulation of several genes (FDR |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect