Isolation and characterization of 8-demethoxy steffimycins and generation of 2,8-demethoxy steffimycins in Streptomyces steffisburgensis by the nogalamycin biosynthesis genes
| Autor: | Jaana Tuikkanen, Anne Hautala, Pekka Mäntsälä, Tero Kunnari, Kristiina Ylihonko, Juha Hakala |
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| Rok vydání: | 1997 |
| Předmět: |
Pharmacology
Antibiotics Antineoplastic Magnetic Resonance Spectroscopy biology Streptomycetaceae Nogalamycin Genes Fungal Wild type Gene Expression Molecular cloning biology.organism_classification Streptomyces Hydroxylation chemistry.chemical_compound Polyketide chemistry Biosynthesis Biochemistry Drug Discovery Anthracyclines Spectrophotometry Ultraviolet Actinomycetales |
| Zdroj: | The Journal of antibiotics. 50(6) |
| ISSN: | 0021-8820 |
| Popis: | Streptomyces steffisburgensis (NRRL 3193, ATCC 27466) is described as a steffimycin producer. Steffimycin belongs to the anthracycline group of aromatic polyketide antibiotics. The structural analysis of the products accumulated by the wild type ATCC 27466 strain revealed three different forms of 8-demethoxy steffimycin suggesting the loss of C-8 hydroxylation/methylation activity. In our approach to generate new anthracycline molecules, we used this strain as a host in gene cloning. The genes encoding the polyketide ketoreductase and aromatase enzymes of nogalamycin biosynthesis caused the production of 2-demethoxy steffimycins in S. steffisburgensis. |
| Databáze: | OpenAIRE |
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