Experimental study of tendon healing early phase: is IGF-1 expression influenced by platelet rich plasma gel?
| Autor: | Dimitrios N Lyras, G. Kiziridis, Anna Kokka, K. Kazakos, Efstathios Chronopoulos, Dionysios Verettas, George Agrogiannis, M. Tryfonidis |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Tendon healing Statistics Nonparametric Immunoenzyme Techniques 03 medical and health sciences Random Allocation 0302 clinical medicine Patellar Ligament medicine Animals Orthopedics and Sports Medicine Insulin-Like Growth Factor I 030222 orthopedics Wound Healing business.industry Platelet-Rich Plasma Patellar ligament Growth factor Histology 030229 sport sciences Immunohistochemistry 3. Good health Tendon Surgery medicine.anatomical_structure Platelet-rich plasma IGF-1 Rabbits Platelet rich plasma business Wound healing Growth factors Gels |
| Zdroj: | Orthopaedicstraumatology, surgeryresearch : OTSR. 96(4) |
| ISSN: | 1877-0568 |
| Popis: | SummaryBackgroundIt is well established that growth factors play a critical role in the healing process of connective tissues. To our knowledge, there are no studies in literature concerning the influence of PRP on growth factors expression.HypothesisThe aim of this study was to assess the effect of a single application of platelet rich plasma (PRP) gel in a patellar tendon defect on the spatial and temporal expression of Insulin-like Growth Factor 1 (IGF-1) during tendon healing.Materials and methodsTwenty-four animals were randomized to receive PRP (PRPFast, Bioteck) in a gel form (PRP group) and 24 to serve as untreated controls (Control group). A defect of 3mm×10mm was surgically created on the tendon under general anaesthetic and in the PRP group, PRP gel was applied to fill the tendon defect whereas no treatment was applied in the control group. Six animals (12 limbs) from each treatment-group were sacrificed after one, two, three and four weeks following treatment. Histological and immunohistochemical staining were performed.ResultsHistology revealed a faster healing process in the tendons of PRP group in comparison with the controls. In the first 2 weeks of healing, IGF-1 was found intracellularly in various type cells, whereas in the last 2 weeks of healing, IGF-1 was detected mainly in tenocytes. Both cytoplasmic and nuclear expressions were present, whereas the larger amounts of immunoexpression were localized in both epitenon and endotenon. A superior expression of IGF-1 was seen in PRP group compared with controls (p |
| Databáze: | OpenAIRE |
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