Vitamin A and galactosyl transferase of tracheal epithelium
| Autor: | George M. Plotkin, George Wolf |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Galactosyltransferase
Male Tracheal Epithelium Vitamin A Deficiency Retinoic acid Retinol General Medicine Retinyl acetate Galactosyltransferases beta-N-Acetylglucosaminylglycopeptide beta-1 4-Galactosyltransferase Uridine Epithelium Rats carbohydrates (lipids) Trachea chemistry.chemical_compound chemistry Biochemistry Microsomes Microsome Transferase Animals lipids (amino acids peptides and proteins) Vitamin A |
| Zdroj: | Biochimica et biophysica acta. 615(1) |
| ISSN: | 0006-3002 |
| Popis: | We developed an assay for galactosyl transferase (uridine diphosphategalactose:glycoprotein galactosyltransferase, EC 2.4.1.22) in microsomes of rat tracheal epithelium and characterized the properties of this enzyme system. We found that vitamin A deficiency greatly depressed galactosyl transferase activity. Activity could be restored within 48 h by dosing the animal with retinyl acetate. Adding retinol to the microsomes from tracheal epithelium of vitamin A-deficient rats also restored galactosyl transferase activity to some extent. Full restoration was achieved by pre-incubating retinol with the microsomal preparation for 30 min. Optimal concentration of pre-incubated retinol was 10−8 M. Pre-incubation with retinyl phosphate and retinoic acid stimulated galactosyl transferase activity in microsomes from tracheas of deficient rats; pre-incubation with dolichyl phosphate, anhydroretinol and the dimethylacetylcyclopentenyl analog of retinoic acid did not. We concluded that vitamin A is involved in the galactosyl transferase system of rat tracheal epithelium, possibly by being linked to galactose. |
| Databáze: | OpenAIRE |
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