Lysolecithin induces demyelination in vitro in a cerebellar slice culture system
| Autor: | Tadimeti S. Rao, Michael Webb, Eric Birgbauer |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Calbindins
Nerve Tissue Proteins Myelin oligodendrocyte glycoprotein Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Myelin S100 Calcium Binding Protein G 2' 3'-Cyclic Nucleotide 3'-Phosphodiesterase Neurofilament Proteins Cerebellum Culture Techniques medicine Animals Regeneration Remyelination Myelin Sheath Neurons biology Phosphoric Diester Hydrolases Multiple sclerosis Oligodendrocyte differentiation Lysophosphatidylcholines Myelin Basic Protein medicine.disease Rats Myelin basic protein Cell biology Myelin-Associated Glycoprotein medicine.anatomical_structure Lysophosphatidylcholine nervous system chemistry biology.protein Myelin-Oligodendrocyte Glycoprotein Neuroscience Myelin Proteins Immunostaining Demyelinating Diseases |
| Zdroj: | Journal of Neuroscience Research. 78:157-166 |
| ISSN: | 1097-4547 0360-4012 |
| Popis: | Demyelination is a hallmark of several human diseases, including multiple sclerosis. To understand better the process of demyelination and remyelination, we explored the use of an in vitro organotypic cerebellar slice culture system. Parasagittal slices of postnatal Day 10 (P10) rat cerebella cultured in vitro demonstrated significant myelination after 1 week in culture. Treatment of the cultures at 7 days in vitro (DIV) with the bioactive lipid lysolecithin (lysophosphatidylcholine) for 15-17 hr in vitro produced marked demyelination. This demyelination was observed by immunostaining for the myelin components myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG), and 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase). After a transient demyelinating insult with lysolecithin in vitro, the cultures recovered with oligodendrocyte differentiation recapitulating a normal time course; there was initially re-expression of CNPase and MBP during this recovery, and this was followed by MOG. In addition, there seemed to be some limited remyelination during the recovery phase. Lysolecithin thus induces demyelination in an in vitro organotypic cerebellar slice culture system, providing a model system for studying myelination, demyelination, and remyelination in vitro. |
| Databáze: | OpenAIRE |
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