Bypassing rRNA methylation by RsmA/Dim1during ribosome maturation in the hyperthermophilic archaeon Nanoarchaeum equitans
| Autor: | Stephen Douthwaite, Henrik Nielsen, Simon Rose, Kenneth H Seistrup, Harald Huber, Ulf Birkedal |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adenosine Ignicoccus Nanoarchaeota/enzymology 030106 microbiology Ribosome Subunits Small Archaeal/metabolism Desulfurococcaceae/enzymology RRNA methylation Ribosome Methylation Methyltransferases/genetics 03 medical and health sciences RRNA modification RNA Ribosomal 16S Genetics Nanoarchaeota Escherichia coli Journal Article Nanoarchaeum equitans Adenosine/metabolism RNA Ribosomal 16S/chemistry biology Desulfurococcaceae Ribosome Subunits Small Archaeal Methyltransferases Ribosomal RNA biology.organism_classification Escherichia coli/genetics 030104 developmental biology Biochemistry Transfer RNA RNA |
| Zdroj: | Seistrup, K H, Rose, S, Birkedal, U, Nielsen, H, Huber, H & Douthwaite, S 2017, ' Bypassing rRNA methylation by RsmA/Dim1during ribosome maturation in the hyperthermophilic archaeon Nanoarchaeum equitans ', Nucleic Acids Research, vol. 45, no. 4, pp. 2007-2015 . https://doi.org/10.1093/nar/gkw839 Nucleic Acids Research |
| Popis: | In all free-living organisms a late-stage checkpoint in the biogenesis of the small ribosomal subunit involves rRNA modification by an RsmA/Dim1 methyltransferase. The hyperthermophilic archaeon Nanoarchaeum equitans, whose existence is confined to the surface of a second archaeon, Ignicoccus hospitalis, lacks an RsmA/Dim1 homolog. We demonstrate here that the I. hospitalis host possesses the homolog Igni_1059, which dimethylates the N(6)-positions of two invariant adenosines within helix 45 of 16S rRNA in a manner identical to other RsmA/Dim1 enzymes. However, Igni_1059 is not transferred from I. hospitalis to N. equitans across their fused cell membrane structures and the corresponding nucleotides in N. equitans 16S rRNA remain unmethylated. An alternative mechanism for ribosomal subunit maturation in N. equitans is suggested by sRNA interactions that span the redundant RsmA/Dim1 site to introduce 2'-O-ribose methylations within helices 44 and 45 of the rRNA. |
| Databáze: | OpenAIRE |
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