Pyrimidine base damage is increased in women with BRCA mutations
| Autor: | Nicoleta C. Voian, Kunle Odunsi, Jean B. Dawidzik, Harold G. Freund, Harold C. Box, Heidi Godoy, Helen B. Patrzyc, Edwin E. Budzinski, Peter J. Frederick |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
DNA damage Genes BRCA2 Genes BRCA1 Biology medicine.disease_cause Article Lesion chemistry.chemical_compound medicine Leukocytes Humans Genetic Predisposition to Disease Mutation BRCA mutation Cancer DNA medicine.disease Molecular biology Thymine Oxidative Stress Pyrimidines Oncology chemistry Case-Control Studies Cancer research Female medicine.symptom Oxidative stress DNA Damage |
| Zdroj: | Cancer letters. 338(2) |
| ISSN: | 1872-7980 |
| Popis: | Oxidatively-induced DNA damage was measured in the DNA of WBC from two groups of women: carriers of a BRCA mutation, but asymptomatic for disease, and healthy controls. Two oxidatively induced lesions were measured: a formamide remnant of pyrimidine base and the glycol modification of thymine. These lesions, employed previously in studies of the effects of smoking, antioxidant usage and ovarian cancer, are proving valuable indicators of oxidative stress. The BRCA carriers of mutations, with no overt sign of cancer, nevertheless had significantly higher levels of DNA damage than the controls. The level measured for the formamide lesion was 5.9 ± 1.0 (femtomoles/μg of DNA ± SEM) compared with 2.4 ± 0.3 in controls. The level of the glycol lesion was 2.9 ± 0.4 compared with 1.8 ± 0.2 in controls. The experimental design utilized DNA from WBC and employed LC–MS/MS to detect the lesions. |
| Databáze: | OpenAIRE |
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