Absence of significant age-dependent increase of single-stranded DNA extracted from mouse liver nuclei
| Autor: | Richard G. Cutler, Roger G. Dean |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Male
Aging Protein Denaturation DNA Single-Stranded Age dependent Biochemistry DNA sequencing chemistry.chemical_compound Mice Endocrinology Genetics medicine Centrifugation Density Gradient Animals Molecular Biology Differential centrifugation Cell Nucleus Nuclease biology Cell Biology Molecular biology Mice Inbred C57BL Cell nucleus medicine.anatomical_structure Isopycnic chemistry Liver biology.protein DNA |
| Zdroj: | Experimental gerontology. 13(5) |
| ISSN: | 0531-5565 |
| Popis: | Possible age-dependent accumulation of physico-chemical changes in purified mouse liver DNA was investigated. Previous reports of another laboratory (Chetsanga et al. , 1977) have indicated the presence and substantial age-dependent increase in the fraction of single-stranded regions in the DNA from three mouse tissues. Using the S 1 nuclease technique for detecting single-stranded DNA sequences, as well as isopycnic density gradient centrifugation and thermal stability analysis, we cannot confirm this result and find no significant change in the double-stranded integrity of purified mouse liver DNA. |
| Databáze: | OpenAIRE |
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