Genomic Sequence Diversity and Population Structure ofSaccharomyces cerevisiaeAssessed by RAD-seq
| Autor: | Catherine L. Ludlow, Angela A. Huang, Justin C. Fay, Gareth A. Cromie, Patrick May, Teresa L. Gilbert, Katie E. Hyma, Aimée M. Dudley, Cecilia Garmendia-Torres |
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| Rok vydání: | 2013 |
| Předmět: |
Heterozygote
Saccharomyces cerevisiae Population Investigations yeast Genome RAD-seq DNA sequencing 03 medical and health sciences 0302 clinical medicine Genotype Genetic variation Genetics education Molecular Biology Genetics (clinical) 030304 developmental biology 2. Zero hunger 0303 health sciences Genetic diversity education.field_of_study Multiple sequence alignment biology Genetic Variation population structure Sequence Analysis DNA genetic diversity biology.organism_classification phylogenetics Phylogeography Genetics Population Genome Fungal 030217 neurology & neurosurgery |
| Zdroj: | G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.113.007492 |
| Popis: | The budding yeast Saccharomyces cerevisiae is important for human food production and as a model organism for biological research. The genetic diversity contained in the global population of yeast strains represents a valuable resource for a number of fields, including genetics, bioengineering, and studies of evolution and population structure. Here, we apply a multiplexed, reduced genome sequencing strategy (restriction site−associated sequencing or RAD-seq) to genotype a large collection of S. cerevisiae strains isolated from a wide range of geographical locations and environmental niches. The method permits the sequencing of the same 1% of all genomes, producing a multiple sequence alignment of 116,880 bases across 262 strains. We find diversity among these strains is principally organized by geography, with European, North American, Asian, and African/S. E. Asian populations defining the major axes of genetic variation. At a finer scale, small groups of strains from cacao, olives, and sake are defined by unique variants not present in other strains. One population, containing strains from a variety of fermentations, exhibits high levels of heterozygosity and a mixture of alleles from European and Asian populations, indicating an admixed origin for this group. We propose a model of geographic differentiation followed by human-associated admixture, primarily between European and Asian populations and more recently between European and North American populations. The large collection of genotyped yeast strains characterized here will provide a useful resource for the broad community of yeast researchers. |
| Databáze: | OpenAIRE |
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