Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis
| Autor: | Diana E. Zamora-Avila, Uziel Castillo-Velázquez, Luis E. Rodríguez-Tovar, Aimé J. Garza-Arredondo, Humberto Rodriguez-Rocha, Victor E. Aguirre-Arzola, Laura M Mar-Solís, Aracely Garcia-Garcia, Adolfo Soto-Domínguez |
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| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
medicine.medical_specialty nutritional composition medicine.drug_class Nutritional composition Anti-Inflammatory Agents Gastroenterology Article Anti-inflammatory Mice bone broth Acetic acid chemistry.chemical_compound R5-920 Internal medicine medicine Animals ulcerative colitis chemistry.chemical_classification Mice Inbred BALB C business.industry Malnutrition Cytokine expression Nutrients General Medicine medicine.disease Ulcerative colitis essential amino acids cytokines Amino acid Disease Models Animal Trinitrobenzenesulfonic Acid chemistry Murine model Cattle Colitis Ulcerative business |
| Zdroj: | Medicina, Vol 57, Iss 1138, p 1138 (2021) Medicina Volume 57 Issue 11 |
| ISSN: | 1648-9144 |
| DOI: | 10.3390/medicina57111138 |
| Popis: | Background and Objectives: Nutritional deficiencies are one of the main triggers for the development of gastrointestinal diseases, such as ulcerative colitis (UC). Therefore, the objective of the present work consisted of determining the nutrients present in the bone broth (BB) and evaluating their anti-inflammatory properties in a murine model of UC, induced by intrarectal administration of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS), and acetic acid (AcOH). The BB was prepared from the femur of bovine cattle and cooked in distilled water for 8 h at 100 ± 2 °C. Materials and Methods: The BB was administered ad libitum to BALB/c mice for 10 days before the induction of UC. Colon samples were collected for histological analysis and determination of cytokine expression levels by qPCR. Results: It was found that amino acids (AA) are the main nutritional contribution of BB, 54.56% of these correspond to essential AA. The prophylactic administration of BB in the murine model of UC reduced histological damage, decreased the expression of IL-1β (61.12%), IL-6 (94.70%), and TNF-α (68.88%), and increased the expression of INF-γ (177.06%), IL-4 (541.36%), and IL-10 (531.97%). Conclusions: This study shows that BB has anti-inflammatory properties, and its consumption can decrease the symptoms of UC. |
| Databáze: | OpenAIRE |
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