Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro
| Autor: | Hui Li, Yuxiang Wang, Xinyang Zhang, Ning Wang, Songsong Xu, Bohan Cheng, Li Leng, Mengqi Wu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty IBMX chicken preadipocyte medicine.medical_treatment Biophysics Peroxisome proliferator-activated receptor Biology Biochemistry cocktail 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine inducer Receptor Molecular Biology chemistry.chemical_classification Original Paper Insulin food and beverages Troglitazone differentiation Cell Biology Original Papers In vitro peroxisome proliferator-activated receptor γ (PPARγ) 030104 developmental biology Endocrinology chemistry Adipogenesis Rosiglitazone medicine.drug |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | Chicken preadipocytes cultured in cocktail supplement with rosiglitazone resulted in a marked increase in lipid droplet accumulation, glycerol-3-phosphate dehydrogenase (GPDH) activity and mRNA expression of adipocyte fatty acid-binding protein (aP2), G0/G1 switch gene 2 (G0S2), peroxisome proliferator-activated receptor γ (PPARγ) and lipolysis. The present study provides a novel induction method for in vitro chicken preadipocyte differentiation. The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation cocktail [insulin (INS), dexamethasone (DEX) and isobutylmethylxanthine (IBMX)] can induce preadipocyte differentiation in mammals, but it is insufficient for chicken (Gallus gallus) adipogenesis. Oleate can induce chicken preadipocyte differentiation, but these differentiated preadipocytes may not be fully functional. The objective of the current study was to evaluate whether chicken preadipocytes can be induced to mature adipocytes by a novel induction method using differentiation cocktail supplemented with PPARγ agonist(s). Chicken preadipocytes cultured in cocktail supplemented with rosiglitazone or troglitazone resulted in a marked increase in lipid droplet accumulation (P |
| Databáze: | OpenAIRE |
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