A multiplexed phospholipid membrane platform for curvature sensitive protein screening
| Autor: | Eider Berganza, Alexander Navarrete, Srivatsan K Vasantham, Alexander Wunsch, Chunting Zhong, Milos Galic, Michael Hirtz, Mirsana P. Ebrahimkutty |
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| Rok vydání: | 2021 |
| Předmět: |
Technology
Materials science Lipid Bilayers Nanotechnology Project 02 engineering and technology Substrate (printing) Curvature Cell membrane 03 medical and health sciences medicine BAR domain General Materials Science Lipid bilayer Phospholipids 030304 developmental biology 0303 health sciences Membranes 2020-024-029290 Cell Membrane (DPN) Membrane Proteins 021001 nanoscience & nanotechnology Membrane medicine.anatomical_structure Membrane curvature 0210 nano-technology ddc:600 Scanning probe lithography IDs [IDs] |
| Zdroj: | Nanoscale, 13 (29), 12642-12650 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/d1nr01133b |
| Popis: | The curvature of lipid membranes plays a key role in many relevant biological processes such as membrane trafficking, vesicular budding and host-virus interactions. In vitro studies on the membrane curvature of simplified biomimetic models in the nanometer range are challenging, due to their complicated nanofabrication processes. In this work, we propose a simple and low-cost platform for curvature sensitive protein screening, prepared through scanning probe lithography (SPL) methods, where lipid bilayer patches of different compositions can be multiplexed onto substrate areas with tailored local curvature. The curvature is imposed by anchoring nanoparticles of the desired size to the substrate prior to lithography. As a proof of principle, we demonstrate that a positive curvature membrane sensitive protein derived from the BAR domain of Nadrin2 binds selectively to lipid patches patterned on substrate areas coated with 100 nm nanoparticles. The platform opens up a path for screening curvature-dependent protein-membrane interaction studies by providing a flexible and easy to prepare substrate with control over lipid composition and membrane curvature. |
| Databáze: | OpenAIRE |
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