Magnesium sulphate prevents lipopolysaccharide-induced cell death in an in vitro model of the human placenta
| Autor: | Cathal McCarthy, Gerard W. O'Keeffe, Louise C. Kenny, Rachel D. Williamson |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Programmed cell death Lipopolysaccharide Cell Survival medicine.medical_treatment Placenta Gene Expression Biology Andrology 03 medical and health sciences chemistry.chemical_compound Magnesium Sulfate 0302 clinical medicine Pregnancy Internal medicine Cell Line Tumor Internal Medicine medicine Humans MTT assay 030212 general & internal medicine Viability assay RNA Messenger 030219 obstetrics & reproductive medicine Cell Death Tumor Necrosis Factor-alpha Obstetrics and Gynecology equipment and supplies Endocrinology Cytokine chemistry Cell culture Tumor necrosis factor alpha Anticonvulsants Female Pyknosis |
| Zdroj: | Pregnancy hypertension. 6(4) |
| ISSN: | 2210-7797 |
| Popis: | Objectives To determine if magnesium sulphate (MgSO 4 ) prevents lipopolysaccharide (LPS)-induced cell death in an in vitro model of the human placenta. Study design BeWo choriocarcinoma cells were treated with increasing concentrations of LPS (10–1000ng/ml) and MgSO 4 ranging from 1 mM to 100 mM for 24 or 48h. For co-treatments, cells were pre-treated with 1mM MgSO 4 for 1h and 200ng/ml LPS was then added for the remaining 48h. To assess viability an MTT assay was carried out along with nuclear staining to assess pyknotic nuclei. The expression of the pro-inflammatory cytokine TNF-α was examined by real-time PCR. Results MgSO 4 had a dose dependent effect of cell viability with a narrow therapeutic window. MgSO 4 (1mM) protected against adverse effects of LPS on cell viability by preventing LPS-induced decreases in cell viability (MTT assay) and protecting against LPS-induced pyknotic changes in nuclear membrane. MgSO 4 also inhibited the elevated TNF-α mRNA expression induced by LPS. Conclusions MgSO 4 preserves BeWo cell viability following an inflammatory insult and reduces the mRNA expression of the inflammatory cytokine TNF-α. These data elucidate a potential therapeutic pathway by which MgSO 4 may be protective in pre-eclampsia. |
| Databáze: | OpenAIRE |
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