Evaluation of an Ion Trap Toxtyper Liquid Chromatography With An Ion Trap Mass Spectrometric Instrument (Toxtyper) for Drug of Abuse Screening in Oral Fluid
| Autor: | Eberhard Wieland, Maria Shipkova, Thomas Plecko, Kevin Berbalk |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Mass spectrometry
01 natural sciences Sensitivity and Specificity Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Limit of Detection medicine Protein precipitation Humans Pharmacology (medical) Sample preparation 030216 legal & forensic medicine Saliva Pharmacology Detection limit Chromatography medicine.diagnostic_test 010401 analytical chemistry 0104 chemical sciences Substance Abuse Detection chemistry Immunoassay Benzoylecgonine Ion trap Buprenorphine medicine.drug Chromatography Liquid |
| Zdroj: | Therapeutic drug monitoring. 40(5) |
| ISSN: | 1536-3694 |
| Popis: | BACKGROUND Oral fluid (OF) is increasingly used as an alternative sample matrix in drug of abuse screening. Screening is commonly performed by immunoassays and results confirmed using laborious gas chromatography-mass spectrometry (GC-MS)-based methods. Therefore, an easy to operate ion trap mass spectrometric (IT-MS) commercial screening method (Toxtyper; Bruker Daltronik, Bremen, Germany) combined with a laboratory-developed sample preparation procedure has been evaluated for their application to OF. METHODS OF samples were subjected to protein precipitation followed by HybridSPE-Phospholipid extraction. Chromatographic separation was achieved by ultra-high-performance liquid chromatography; MS2/MS3 spectra were recorded by IT-MS and analyzed using a library provided by the manufacturer (Bruker Daltronik). The lower limit of detection, linearity, imprecision, inaccuracy, and specificity (interferences and matrix effects) were investigated for methadone, buprenorphine, pregabalin, fentanyl, amphetamine, 3,4-methylendioxy-N-methylamphetamine, cocaine, acetylcodeine, and nordiazepam, after spiking drug-free OF with these test substances. In addition, concordance between IT-MS results and gas chromatography-tandem mass spectrometry, liquid chromatography-tandem mass spectrometry, or immunoassay (buprenorphine) results was investigated. RESULTS No interferences or matrix effects were observed. The lower limit of detection for acetylcodeine, amphetamine, benzoylecgonine, methadone, and nordiazepam was below the common cutoffs for immunological screening assays and comparable to that of GC-MS. Imprecision and inaccuracy, both in- and between-series, were consistently |
| Databáze: | OpenAIRE |
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