Routine Titrations of Lansing Virus and Antibody in Mice: Comparison of Methods of Estimating Endpoints

Autor:	A. J. Rhodes, D. B. W. Reid, Eina M. Clark
Rok vydání:	1952
Předmět:	Chromatography biology Chemistry Immunoglobulins Gamma globulin Antibodies General Biochemistry Genetics and Molecular Biology Poliomyelitis virus Mice Poliovirus Standard error Immunology biology.protein Animals Titration Antibody Poliomyelitis Lansing Virus
Zdroj:	Experimental Biology and Medicine. 81:118-121
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-81-19796
Popis:	Summary1. Over a period of several months, routine titrations in mice were carried out by a single worker of a pool of Lansing virus, and of samples of plasma gamma globulin, placental gamma globulin, and immune monkey serum. Estimates of the ED50's and their standard errors were calculated for the individual titrations by the probit method. In addition ED50's were calculated by the Karber, Reed and Muench, and moving average methods. 2. The standard deviation of the titrations was found to be greater than the error of individual assays, suggesting that titrations carried out on different occasions vary more than those performed at the same time. 3. None of the methods mentioned gave widely different estimates of the ED50. In particular, the ED50's calculated by the Karber method compared very favourably with those obtained by the probit method. 4. The Karber method is proposed as a suitable alternative to the Reed and Muench procedure for the calculation of ED50 in routine titrations of virus and antiserum.
Databáze:	OpenAIRE
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