Tamm-Horsfall glycoprotein (THG) purified from normal human pregnancy urine increases phagocytosis, complement receptor expressions and arachidonic acid metabolism of polymorphonuclear neutrophils
| Autor: | Chia-Li Yu, Whu Mei Lin, Kuang Hui Sun, Tang Shueen Liao, Chang-Youh Tsai, Kuen Horng Chen |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Tamm–Horsfall protein Neutrophils Phagocytosis Macrophage-1 Antigen Enzyme-Linked Immunosorbent Assay Complement receptor Dinoprostone Membrane Potentials chemistry.chemical_compound Mucoproteins Complement Receptor Type 1 Pregnancy Internal medicine Uromodulin medicine Humans Prostaglandin E2 Receptor Pharmacology Arachidonic Acid Binding Sites biology Flow Cytometry Receptors Complement Thromboxane B2 Endocrinology chemistry biology.protein Arachidonic acid Electrophoresis Polyacrylamide Gel Female medicine.drug |
| Zdroj: | Immunopharmacology. 24(3) |
| ISSN: | 0162-3109 |
| Popis: | Tamm-Horsfall glycoprotein (THG) purified from normal human pregnancy urine was found to increase polymorphonuclear neutrophil (PMN) phagocytosis (46.57 +/- 3.54% in the medium versus 75.85 +/- 5.37% in the presence of 25 micrograms/ml THG) after 30 min preincubation. The phagocytosis-enhancing activity of THG was dose-dependent (5-50 micrograms/ml) and was possibly mediated by the increased expressions of complement receptor type 1 (CR1) and type 3 (CR3) on the neutrophils. The release of [3H]arachidonic acid and prostaglandin E2 (PGE2), but not thromboxane B2 (TXB2), from neutrophils were also significantly enhanced by THG. Using 3,3'-dihexyloxacarbocyanine iodide as indicator, THG (25 micrograms/ml) depolarized the membrane potential of PMN after 30 min preincubation. In addition, THG exhibited a specific membranotropic effect with PMN. It is conceivable that THG binds to the cell surface and depolarizes the membrane potential of PMN which subsequently enhances the release of arachidonic acid metabolites and the translocation of the complement receptors to the membrane. These biochemical events lead to the increment of PMN phagocytosis and suggests that THG may play an important role in the defense mechanisms of the urinary tract in that a large amount of THG is usually present. |
| Databáze: | OpenAIRE |
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