Prolonged sampling of spontaneous sputum improves sensitivity of hypermethylation analysis for lung cancer
| Autor: | Henk Smit, Erik Thunnissen, Peter J.F. Snijders, A. Jasmijn Hubers, Pieter E. Postmus, Sylvia Duin, Egbert F. Smit, Peter J. Sterk, Peter W.A. Kunst, Birgit I. Witte, Sjaak Burgers, Gerarda J.M. Herder, Daniëlle A.M. Heideman |
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| Přispěvatelé: | Amsterdam institute for Infection and Immunity, Pulmonology, Pathology, Pulmonary medicine, Epidemiology and Data Science, CCA - Oncogenesis |
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Sputum Cytology Pathology Lung Neoplasms Time Factors Colorectal cancer Adenomatous polyposis coli Adenomatous Polyposis Coli Protein Gastroenterology Specimen Handling Pathology and Forensic Medicine Pulmonary Disease Chronic Obstructive Predictive Value of Tests Internal medicine medicine Humans Prospective Studies Lung cancer Prospective cohort study Aged biology business.industry Tumor Suppressor Proteins Cytoglobin Sputum DNA Neoplasm General Medicine DNA Methylation medicine.disease Globins Predictive value of tests biology.protein Female medicine.symptom business |
| Zdroj: | Hubers, A J, Heideman, D A M, Herder, G J M, Burgers, S A, Sterk, P J, Kunst, P W, Smit, H J, Postmus, P E, Witte, B I, Duin, S, Snijders, P J F, Smit, E F & Thunnissen, F B J M 2012, ' Prolonged sampling of spontaneous sputum improves sensitivity of hypermethylation analysis for lung cancer ', Journal of Clinical Pathology, vol. 65, no. 6, pp. 541-545 . https://doi.org/10.1136/jclinpath-2012-200712 Journal of clinical pathology, 65(6), 541-545. BMJ Publishing Group Journal of Clinical Pathology, 65(6), 541-545. BMJ Publishing Group |
| ISSN: | 1472-4146 0021-9746 |
| Popis: | Aims The adequacy of lung cancer diagnosis with sputum cytology depends on duration of sputum sampling. The aim of this methodological study was to determine whether the hypermethylation detection rate of RASSF1A, adenomatous polyposis coli (APC) and cytoglobin (CYGB) is influenced by the duration of sputum collection. Methods Prospective sputum samples were collected from 53 lung cancer patients and 47 chronic obstructive pulmonary disease patients as controls. Subjects collected spontaneous sputum at home during nine consecutive days in three canisters I, II and III (ie, days 1–3, days 4–6, days 7–9, respectively). Quantitative methylation-specific PCR was performed to assess gene promoter methylation status of RASSF1A, APC and CYGB. Results Analysis of each canister separately showed hypermethylation of RASSF1A, APC and/or CYGB in samples I, II and III, in 43%, 40% and 47% of cases, respectively. In control samples, these numbers were 4%, 2% and 4%, respectively. Cumulative analysis for days 1–6 and days 1–9 revealed an increase in sensitivity to 53% and 64%, and specificity of 94% and 91%, respectively. Conclusion Sputum collected over multiple successive days results in a gain in sensitivity for the detection of lung cancer, at the expense of a small loss in specificity. Condensed abstract Assessment of hypermethylation sensitivity of biomarkers in sputum collected over a prolonged period for the detection of lung cancer resulted in a promising gain in sensitivity, at the expense of a small loss in specificity. |
| Databáze: | OpenAIRE |
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