Low-temperature laminar flow ward for the treatment of multidrug resistance Acinetobacter baumannii pneumonia

Autor: Zhidong Hu, Daqiang Zhan, Jinhao Huang, Xuanhui Liu, Wei Quan, Rongcai Jiang, Jian Sun, Chuang Gao, Hongliang Luo, Ye Tian, Jin Li, Zhitao Gong, Yu Qian, Shuo An, Hengjie Yuan, Yiming Song
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Acinetobacter baumannii
Male
Antibiotics
Drug resistance
law.invention
0302 clinical medicine
Medical microbiology
law
Drug Resistance
Multiple
Bacterial
030212 general & internal medicine
biology
Virulence
Temperature
General Medicine
Middle Aged
Environment
Controlled
Intensive care unit
Anti-Bacterial Agents
Cold Temperature
Intensive Care Units
Infectious Diseases
Treatment Outcome
Original Article
Female
Acinetobacter Infections
Microbiology (medical)
medicine.medical_specialty
medicine.drug_class
Antibiotic sensitivity
030106 microbiology
Variation
Microbial Sensitivity Tests
03 medical and health sciences
Antibiotic resistance
Internal medicine
Patients' Rooms
medicine
Pneumonia
Bacterial
Humans
Aged
Retrospective Studies
Microbial Viability
business.industry
Pneumonia
biology.organism_classification
Multiple drug resistance
Treatment
business
Zdroj: European Journal of Clinical Microbiology & Infectious Diseases
ISSN: 1435-4373
0934-9723
Popis: This study was designed to investigate the effect of low-temperature laminar flow ward (LTLFW) on the Acinetobacter baumannii pneumonia (MDR-ABP) in neurosurgical intensive care unit (NICU) patients. We evaluated whether patients in a LTLFW had significantly improved clinical outcomes as compared to those in nonconstant-temperature NICU (room temperature). The association of temperature with the prevalence of ABP and A. baumannii isolates (ABI) found in NICU patients was specifically investigated. In vitro microbiological experiments were conducted to measure the proliferation, antibiotic sensitivity, and genomic profiles of A. baumannii (AB) that grew in variable temperatures. MDR-ABP patients in LTLFW had significantly improved outcomes than those in the room temperature NICU. In addition, the numbers of ABI were positively associated with mean ambient outdoor temperatures (P = 0.002), with the incidence of ABP and average numbers of ABI among NICU patients being substantially lower in the winter as compared to other seasons. However, there were no significant seasonal variations in the other strains of the top five bacteria. Consistent with these clinical observations, AB growing at 20°C and 25°C had significantly reduced viability and antibiotic resistance compared to those growing at 35°C. The expression of genes related to AB survival ability, drug resistance, and virulence also differed between AB growing at 20°C and those at 35°C. LTLFW is effective in promoting the recovery of MDR-ABP patients because low temperatures reduced the density and virulence of AB and enhanced the efficacy of antibiotics, likely at the genetic level. Electronic supplementary material The online version of this article (10.1007/s10096-019-03790-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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