Low-temperature laminar flow ward for the treatment of multidrug resistance Acinetobacter baumannii pneumonia
Autor: | Zhidong Hu, Daqiang Zhan, Jinhao Huang, Xuanhui Liu, Wei Quan, Rongcai Jiang, Jian Sun, Chuang Gao, Hongliang Luo, Ye Tian, Jin Li, Zhitao Gong, Yu Qian, Shuo An, Hengjie Yuan, Yiming Song |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Acinetobacter baumannii Male Antibiotics Drug resistance law.invention 0302 clinical medicine Medical microbiology law Drug Resistance Multiple Bacterial 030212 general & internal medicine biology Virulence Temperature General Medicine Middle Aged Environment Controlled Intensive care unit Anti-Bacterial Agents Cold Temperature Intensive Care Units Infectious Diseases Treatment Outcome Original Article Female Acinetobacter Infections Microbiology (medical) medicine.medical_specialty medicine.drug_class Antibiotic sensitivity 030106 microbiology Variation Microbial Sensitivity Tests 03 medical and health sciences Antibiotic resistance Internal medicine Patients' Rooms medicine Pneumonia Bacterial Humans Aged Retrospective Studies Microbial Viability business.industry Pneumonia biology.organism_classification Multiple drug resistance Treatment business |
Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases |
ISSN: | 1435-4373 0934-9723 |
Popis: | This study was designed to investigate the effect of low-temperature laminar flow ward (LTLFW) on the Acinetobacter baumannii pneumonia (MDR-ABP) in neurosurgical intensive care unit (NICU) patients. We evaluated whether patients in a LTLFW had significantly improved clinical outcomes as compared to those in nonconstant-temperature NICU (room temperature). The association of temperature with the prevalence of ABP and A. baumannii isolates (ABI) found in NICU patients was specifically investigated. In vitro microbiological experiments were conducted to measure the proliferation, antibiotic sensitivity, and genomic profiles of A. baumannii (AB) that grew in variable temperatures. MDR-ABP patients in LTLFW had significantly improved outcomes than those in the room temperature NICU. In addition, the numbers of ABI were positively associated with mean ambient outdoor temperatures (P = 0.002), with the incidence of ABP and average numbers of ABI among NICU patients being substantially lower in the winter as compared to other seasons. However, there were no significant seasonal variations in the other strains of the top five bacteria. Consistent with these clinical observations, AB growing at 20°C and 25°C had significantly reduced viability and antibiotic resistance compared to those growing at 35°C. The expression of genes related to AB survival ability, drug resistance, and virulence also differed between AB growing at 20°C and those at 35°C. LTLFW is effective in promoting the recovery of MDR-ABP patients because low temperatures reduced the density and virulence of AB and enhanced the efficacy of antibiotics, likely at the genetic level. Electronic supplementary material The online version of this article (10.1007/s10096-019-03790-x) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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