Extracellular Recordings From Patterned Neuronal Networks Using Planar Microelectrode Arrays
| Autor: | Andrew J. Spence, K.L. Smith, Conrad D. James, Harold G. Craighead, William Shain, Rifat J. Hussain, Michael Isaacson, Natalie Dowell-Mesfin, James N. Turner |
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| Rok vydání: | 2004 |
| Předmět: |
Materials science
Neurite Cell Culture Techniques Biomedical Engineering Action Potentials Biocompatible Materials Hippocampal formation Hippocampus Haptotaxis Rats Sprague-Dawley Bursting Extracellular Animals Cells Cultured Neurons Equipment Design Multielectrode array Rats Electrophysiology Equipment Failure Analysis Microelectrode Microcontact printing Biophysics Nerve Net Extracellular Space Microelectrodes Neuroscience |
| Zdroj: | IEEE Transactions on Biomedical Engineering. 51:1640-1648 |
| ISSN: | 0018-9294 |
| Popis: | Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (microCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-microm-wide lines spaced by 50-200 microm with 15-25-microm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a approximately 2 microm bundle of guided neurites. |
| Databáze: | OpenAIRE |
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