Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil
| Autor: | Celso Francisco Hernandes Granato, Aripuanã Sakurada Aranha Watanabe, Emerson Carraro, Marcel Leite, Rita de Cássia Compagnoli Carmona |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Diarrhea Male Rotavirus lcsh:Arctic medicine. Tropical medicine Adolescent Genotype lcsh:RC955-962 Epidemiology 030106 microbiology Biology medicine.disease_cause Chromatography Affinity Rotavirus Infections 03 medical and health sciences Young Adult medicine Humans Latex agglutination assay Child Genotyping Phylogeny Infant Newborn Infant General Medicine Middle Aged Virology 030104 developmental biology Infectious Diseases Child Preschool Geographic regions Etiology Immunization program RNA Viral Original Article Female Seasons Vaccine Brazil |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo, Volume: 59, Article number: e45, Published: 03 AUG 2017 Revista do Instituto de Medicina Tropical de São Paulo Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 59 (2017); e45 Revista do Instituto de Medicina Tropical de São Paulo; v. 59 (2017); e45 Instituto de Medicina Tropical (IMT) instacron:IMT |
| ISSN: | 1678-9946 0036-4665 |
| Popis: | Rotavirus is the main global cause of severe childhood diarrhoea among children. In 2006, Rotarix® (G1P[8]) was introduced into Brazil’s National Immunization Program. The vaccine coverage rate was 84.4% in 2009. Evidences of increasing G2P[4] after 2006 opened up the discussion about the vaccine effectiveness to non-G1 strains. The aim of this study was to identify the circulating rotavirus genotypes in two Brazilian regions during 2009. A total of 223 positive samples by immunochromatography and latex agglutination assay from the Northeast (Bahia/Pernambuco States) and Southeast (São Paulo/Rio de Janeiro States) regions were included in the study. The samples were submitted to genotyping by nested-PCR according to VP7(G) and VP4(P) and 175 samples (78.5%) were able to be characterized. Considering the characterization of VP7, the G-types detected were G1, G2, and G4 in the Northeast, and G2, G3, G5, and G9 in the Southeast. Considering the characterization of VP4, the P-types detected were P[4], P[8], and P[6]/P[9] in the Northeast and the Southeast. The most frequent mixed types found were G2P[4]/G2P[NT](81.4%), G2P[6](5.2%), G1P[6](5.2%) in the Northeast, and G2P[4]/G2P[NT](78.8%), G2P[6](8.2%), G9P[8](4.7%) in the Southeast. Among immunized individuals whose age ranged from 0-4 years, the G2P[4]/G2P[NT] genotype was identified in 91,0% of cases, and among non-immunized individuals of the same age, the G2P[4]/G2P[NT] genotype was identified in 85.7% of the cases. In accordance with the high level of vaccine coverage, the data suggest that the circulation of G2P[4] in these regions had a considerable increase after the introduction of Rotarix®. |
| Databáze: | OpenAIRE |
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