Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans
| Autor: | Rolf A. Prade, Stephen G. Oliver, Hugh H. Russell, Andrew H. Sims, Manda E. Gent, David C. Hoyle, Geoffrey Turner, Nigel Dunn-Coleman, Geoffrey D. Robson |
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| Rok vydání: | 2004 |
| Předmět: |
Microarray
Genes Fungal Microbiology Aspergillus nidulans Fungal Proteins Gene Expression Regulation Fungal Genetics Genomic library DNA Fungal Gene Library Oligonucleotide Array Sequence Analysis Expressed Sequence Tags Expressed sequence tag Ethanol biology Microarray analysis techniques Gene Expression Profiling Gluconeogenesis Glyoxylates Reproducibility of Results biology.organism_classification Yeast Culture Media Gene expression profiling Glucose DNA microarray |
| Zdroj: | Fungal Genetics and Biology. 41:199-212 |
| ISSN: | 1087-1845 |
| DOI: | 10.1016/j.fgb.2003.11.005 |
| Popis: | The use of microarrays in the analysis of gene expression is becoming widespread for many organisms, including yeast. However, although the genomes of a number of filamentous fungi have been fully or partially sequenced, microarray analysis is still in its infancy in these organisms. Here, we describe the construction and validation of microarrays for the fungus Aspergillus nidulans using PCR products from a 4092 EST conidial germination library. An experiment was designed to validate these arrays by monitoring the expression profiles of known genes following the addition of 1% (w/v) glucose to wild-type A. nidulans cultures grown to mid-exponential phase in Vogel's minimal medium with ethanol as the sole carbon source. The profiles of genes showing statistically significant differential expression following the glucose up-shift are presented and an assessment of the quality and reproducibility of the A. nidulans arrays discussed. |
| Databáze: | OpenAIRE |
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